Ukuhlaziywa kwe-Bulked Segregant
Izinzuzo Zesevisi
Takagi et al., Ijenali yezitshalo, 2013
● Ukwenza kwasendaweni okunembile: Ukuhlanganisa inqwaba nabantu abangu-30+30 kuya ku-200+200 ukuze unciphise umsindo ongemuva;isibikezelo sesifunda sekhandidethi elisuselwa ku-mutatants okungelona igama elifanayo.
● Ukuhlaziya okuphelele: Isichasiselo somsebenzi wofuzo wekhandidethi ojulile, ohlanganisa i-NR, i-SwissProt, i-GO, i-KEGG, i-COG, i-KOG, njll.
● Isikhathi sokushintsha esisheshayo: Ukwenziwa kwasendaweni ngokushesha kwezakhi zofuzo zingakapheli izinsuku zokusebenza ezingama-45.
● Okuhlangenwe nakho okubanzi: I-BMK inikele ngezinkulungwane zezici zokwenziwa kwasendaweni, ezihlanganisa izinhlobo ezihlukahlukene ezifana nezitshalo, imikhiqizo yasemanzini, ihlathi, izimbali, izithelo, njll.
Imininingwane Yesevisi
Inani labantu:
Ukuhlukanisa inzalo yabazali abane-phenotypes ephikisanayo.
isib. Inzalo ye-F2, i-Backcrossing (BC), i-Recombinant inbred line(RIL)
Ichibi lokuxuba
Ngezici zekhwalithi: abantu abangama-30 kuye kwangama-50(ubuncane abangama-20)/ngobuningi
Ku-tratis yobuningi: abantu abaphezulu abangu-5% kuya ku-10% abanama-phenotypes adlulele kuso sonke isibalo sabantu (ubuncane obungu-30+30).
Ukujula kokulandelana okunconyiwe
Okungenani u-20X/umzali kanye no-1X/inzalo yomuntu ngamunye (isb. echibini lokuxuba abantwana labangu-30+30, ukujula kokulandelana kuzoba ngu-30X ngenqwaba)
I-Bioinformatics ihlaziya
● Ukulandelana kofuzo lonke
● Ukucubungula idatha
● Ukushaya kwe-SNP/Indel
● Ukuhlolwa kwesifunda kwamakhandidethi
● Isichasiselo somsebenzi wofuzo wekhandidethi
Izidingo Zesampula Nokulethwa
Izimfuneko zesampula:
Ama-Nucleotides:
| isampula ye-gDNA | Isampula yezicubu |
| Ukugxila: ≥30 ng/μl | Izitshalo: 1-2 g |
| Inani: ≥2 μg (Ivolumu ≥15 μl) | Izilwane: 0.5-1 g |
| Ubumsulwa: OD260/280= 1.6-2.5 | Igazi eliphelele: 1.5 ml |
Ukugeleza Komsebenzi Wesevisi
Idizayini yokuhlola
Ukulethwa kwesampula
Ukukhishwa kwe-RNA
Ukwakhiwa komtapo wolwazi
Ukulandelanisa
Ukuhlaziywa kwedatha
Amasevisi angemuva kokuthengisa
1.Isisekelo sokuhlaziya i-Association ku-Euclidean Distance (ED) ukuze kuhlonzwe isifunda sekhandidethi.Emfanekisweni olandelayo
I-X-axis: Inombolo ye-Chromosome;Ichashazi ngalinye limelela inani le-ED le-SNP.Ulayini omnyama uhambisana nevelu ye-ED efakiwe.Inani eliphakeme le-ED libonisa ukuhlobana okubaluleke kakhulu phakathi kwesayithi kanye ne-phenotype.Umugqa wedeshi obomvu umele umkhawulo wokuhlobana okubalulekile.
2.Ukuhlaziywa kwenhlangano okusekelwe kunkomba ye-SNP
I-X-axis: Inombolo ye-Chromosome;Ichashazi ngalinye limelela inani lenkomba ye-SNP.Ulayini omnyama umele inani lenkomba ye-SNP efakiwe.Uma inani lilikhulu, ukuhlangana kubaluleke kakhulu.
Icala le-BMK
I-ain-effect quantitative trait locus Fnl7.1 ifaka amaprotheni amaningi e-embryogenesis ehambisana nobude bentamo yesithelo kukhukhamba.
Kushicilelwe: Plant Biotechnology Journal, 2020
Isu lokulandelanisa:
Abazali (Jin5-508, YN): Lonke igenome elandelanayo kokungu-34× no-20×.
Amachibi e-DNA (ama-50 anentamo emide nama-50 anentamo emfushane): Ilandelanisa i-61× kanye ne-52×
Imiphumela esemqoka
Kulolu cwaningo, ukuhlukaniswa kwabantu(F2 kanye ne-F2:3) kwenziwe ngokuwela umugqa wekhukhamba wentamo ende i-Jin5-508 kanye nentamo emfushane YN.Amachibi amabili e-DNA akhiwa abantu abangu-50 abanezintamo ezinde ngokwedlulele kanye nabangama-50 abanezintamo ezimfishane ngokwedlulele.Umthelela omkhulu we-QTL uhlonzwe ku-Chr07 ngokuhlaziywa kwe-BSA kanye nokumepha okungokwesiko kwe-QTL.Isifunda sekhandidethi siphinde sancishiswa ngokudwetshwa kwemephu okuhle, ukulinganisa isisho sofuzo kanye nokuhlolwa kwe-transgenic, okuveze isakhi sofuzo esibalulekile ekulawuleni ubude bentamo, i-CsFnl7.1.Ngaphezu kwalokho, i-polymorphism endaweni yomgqugquzeli we-CsFnl7.1 itholwe ihlotshaniswa nenkulumo ehambisanayo.Ukuhlaziywa okwengeziwe kwe-phylogenetic kuphakamise ukuthi i-Fnl7.1 locus kungenzeka kakhulu ukuthi idabuka eNdiya.
 I-QTL-mapping ekuhlaziyweni kwe-BSA ukuhlonza isifunda sekhandidethi esihlotshaniswa nobude bentamo yekhukhamba |  Amaphrofayili e-LOD e-QTL yobude bentamo yekhukhamba akhonjwe ku-Chr07 |
Xu, X., et al."Umphumela omkhulu we-quantitative trait locus Fnl7.1 ufaka iphrotheni eningi ye-embryogenesis ehambisana nobude bentamo yesithelo kukhukhamba."Ijenali ye-Plant Biotechnology 18.7(2020).
