GENOME EVOLUTION
Genome De novo
Gungano | Kutsunga bonde Iwo ese ekutevedzana mabasa uye chikamu chebioinformatic masevhisi akapihwa neBiomarker Techonologies.
Abstract
“Chiratidzo chezvidhori zvegungwa chinosanganisira mashizha akaita semashizha, muromo usina mazino, uye pamuviri wemurume unosanganisira kuvharirwa mazai pa "brood patch" yakashama.We de novo-yakatevedzana echirume neechikadzi genomes eyakajairwa seadragon (Phyllopteryx taeniolatus) nemhando dzayo dzine hukama, alligator pipefish (Syngnathoides biaculeatus).Zvinyorwa zvinyorwa kubva kutsvarakadenga, mashizha-akafanana nemaappendages, anoratidza kuti seti yemajini inowanzobatanidzwa mukuvandudza kwekupedzisira yakasarudzwa pamwe chete nekupfumisa zvinyorwa zvekugona kugadzirisa matishu uye majini ekudzivirira immune.Izebrafish mutants ye scpp5, iyo yakarasika mune zvose syngnathids, yakawanikwa isina kana kuti yakasvibiswa mazino epharyngeal, ichitsigira pfungwa yokuti kurasikirwa kwe scpp5 kwakakonzera kurasikirwa kwemazino muma syngnathids.Iyo yekuisa bonde-inosarudzira nzvimbo inokodha yemurume-yakananga amhr2y gene yakagovaniswa neyakajairika seadragon uye alligator pipefish yakaonekwa. ”
Zvishandiso uye nzira:
Zvishandiso
Fgungwa redu rakafanana (P. taeniolatus) nealligator pipefish mbiri (S. biaculeatus) mienzaniso.Maviri seadragoni akajairika (mumwe murume nemukadzi mumwe) uye maviri alligator pipefish (mumwe murume nemurume mumwe chete) vanhu vakashandiswa kutevedzera-genome sequencing, uye mamwe maviri echirume akajairika seadragon vanhu vakashandiswa kuita genome resequencing.
Genome Sequencing strategy
270/350 bp raibhurari (Hiseq 2500) + Pacbio 20Kb (Sequel) kana Nanopore 30 Kb (MinION)+ Hi-C (NovaSeq 6000) .sequencing, uye vamwe vaviri vanhurume common seadragon vanhu vakashandiswa genome resequencing.
Transcriptome Sequencing
Fkana common seadragon, uropi, ziso, gill, chiropa, mwoyo, itsvo, mesentery, ura, tsandanyama, fin, ganda, mashizha-akafanana nemashizha, testis, uye ovary zvakaunganidzwa.
Genome de novo assembly strategy
Canu (Kururamisa) + WTDBG2 (Assembly) + Pilon(Polish) + Lachesis (Hi-C).
Main Results
Fig. 1. Mamiriro akakosha eiyo common seadragon (P. taeniolatus) uye alligator pipefish (S. biaculeatus) nenzvimbo dzadzo dze phylogenetic.
Fig. 2. Morphology uye genetic makeup yemashizha-se-appendages mujojo yegungwa inowanikwa (P. taeniolatus).
Fig. 3. Putative sex determination gene in the common seadragon (P. taeniolatus) nealligator pipefish (S. biaculeatus).
Mufananidzo 4. Pharyngeal zino phenotypes mu zebrafish scpp5 homozygous mutants.
Nguva yekutumira: Jan-08-2022