HORUMARINTA GENOME
Genome De novo
Golaha
Abstract
"Nooca astaanta u ah sedragons-ka badda waxaa ka mid ah lifaaqyo caleen u eg, af tubbo leh oo aan ilig lahayn, iyo uur lab ah oo ku lug leh ukunta la bacrimiyey oo ku taal meel furan.Waxaan de novo-isku xigxiga labka iyo dheddigga genome ee badmareenka caadiga ah (Phyllopteryx taeniolatus) iyo noocyada ay si dhow ula xiriiraan, alligator pipefish (Syngnathoides biculeatus).Qoraallada qoraallada laga soo qaatay horumar cusub, lifaaqyada caleen u eg, ayaa muujinaya in hiddo-wadeyaal sida caadiga ah ku lug leh horumarinta finka la doortay iyo sidoo kale kobcinta qoraallada tran si loo dayactiro unugyada suurtagalka ah iyo hiddo-wadaha difaaca difaaca.Mutants zebrafish for scpp5, kaas oo ka lumay dhammaan syngnathids, ayaa la ogaaday inay ka maqan yihiin ama ay qalloocmeen ilkihii hunguriga, taageeraya fikradda ah in luminta scpp5 ay gacan ka geysatay luminta ilkaha syngnathids.Galmo fiiqan-go'aaminaya meesha codeeya hidde-sidaha amhr2y ee lab-gaar ah ee ay wadaagaan badeedka caadiga ah iyo pipefish aligator ayaa la aqoonsaday. ”
Qalabka iyo hababka:
Qalabka
Fbadmareenkeena guudP. taeniolatusiyo laba kalluun alligator ah (S. biculeatus) muunado.Laba badmareen oo caadi ah (hal lab iyo hal dhedig) iyo laba kalluun alligator ah (hal lab iyo hal dhedig) shakhsiyaad ayaa loo adeegsaday isku xigxiga guud ahaan-genome, iyo laba kale oo lab ah shakhsiyaadka badmareenka caadiga ah ayaa loo adeegsaday dib-u-soo-celinta genome.
Istaraatiijiyada Taxanaha Genome
270/350 bp maktabadda (Hiseq 2500) + Pacbio 20Kb (Taxane) ama Nanopore 30 Kb (MinION)+ Hi-C (NovaSeq 6000) .isku xigxiga, iyo laba kale oo lab ah shakhsiyaadka badmareenka caadiga ah ayaa loo isticmaalay isku-dabaynta genome.
Taxanaha qoraalka qoraalka
Fama badmareenada caadiga ah, maskaxda, isha, beerka, beerka, wadnaha, kelyaha, mesentery, xiidmaha, muruqa, fin, maqaarka, lifaaqyada caleen u eg, xiniinyaha, iyo ugxansidaha ayaa la ururiyey.
Genome de novo shirka
Canu (Sixitaan) + WTDBG2 (Golaha) + Pilon (Polish) + Lachesis (Hi-C).
Natiijooyinka ugu muhiimsan
Jaantuska. 1. Tilmaamaha muhiimka ah ee badmareenka caadiga ah (P. taeniolatus) iyo pipefish alligator (S. biculeatus) iyo boosaskooda phylogenetic.
Jaantuska. 2. Morfology iyo qurxinta hidda-socodka ee lifaaqyada caleen u eg ee badmareenka caadiga ah (P. taeniolatus).
Jaantuska. 3. Hiddaha go'aaminta jinsiga ee ku jira badmareenada caadiga ah (P. taeniolatus) iyo pipefish alligator (S. biaculeatus).
Jaantuska. 4. Noocyada phenotypes iliga pharyngeal ee zebrafish scpp5 mutants homozygous.
Waqtiga boostada: Jan-08-2022