I-GENOME EVOLUTION
I-Genome De novo
I-Assembly|Ukuzimisela ngobulili Yonke imisebenzi yokulandelanisa kanye nengxenye yezinsizakalo ze-bioinformatic yanikezwa yi-Biomarker Techonologies.
Abstract
“I-phenotype eyisakhiwo sodumo yamadragoni asolwandle ihlanganisa ama-appendages afana namaqabunga, umlomo oshubhu ongenamazinyo, nokukhulelwa kwabesilisa okuhilela ukufukamela amaqanda avundisiwe “esiqiwini” esivulekile.Silandelanisa ama-genome owesilisa nowesifazane we-seadragon evamile (Phyllopteryx taeniolatus) kanye nezinhlobo zayo ezihlobene eduze, i-alligator pipefish (Syngnathoides biaculeatus).Amaphrofayli alotshiweyo avela entweni entsha, ama-appendages afana neqabunga, abonisa ukuthi isethi yezakhi zofuzo ngokuvamile ezibandakanyekayo ekuthuthukisweni kokugcina zikhethiwe kanye nokunothiswa kwemibhalo ebhaliwe yokulungisa izicubu ezingaba khona kanye nezakhi zofuzo zokuzivikela komzimba.I-zebrafish mutants ye-scpp5, elahlekile kuwo wonke ama-sygnathids, atholakale engenawo noma anamazinyo akhubazekile e-pharyngeal, asekela umbono wokuthi ukulahlekelwa kwe-scpp5 kube nomthelela ekulahlekeni kwamazinyo kuma-sygnathids.I-putive sex-determining locus efaka isakhi sofuzo se-amhr2y esiqondene nomuntu wesilisa esabelwa i-seadragon evamile kanye ne-alligator pipefish ikhonjiwe. ”
Izimpahla nezindlela:
Izinto zokwakha
Fudrako wethu ovamile (P. taeniolatus) kanye ne-alligator pipefish (S. biaculeatus) izibonelo.Ama-seadragon amabili avamile (owesilisa oyedwa nowesifazane oyedwa) kanye ne-alligator pipefish (oyedwa owesilisa noyedwa wesifazane) asetshenziselwa ukulandelana kwezakhi zofuzo eziphelele, kanti abanye ababili besilisa abavamile be-seadragon basetshenziselwa ukulandelana kofuzo.
I-Genome Sequencing isu
2Ilabhulali engu-70/350 bp (Hiseq 2500) + Pacbio 20Kb (I-Sequel) noma i-Nanopore 30 Kb (MinION)+ Hi-C (NovaSeq 6000).ukulandelana, kanye nabanye ababili besilisa abavamile be-seadragon ngabanye basetshenziselwa ukulandelana kofuzo.
Ukulandelana kwe-Transcriptome
Fnoma udrako ovamile wasolwandle, ubuchopho, iso, igill, isibindi, inhliziyo, izinso, i-mesentery, amathumbu, imisipha, i-fin, isikhumba, ama-appendages afana namaqabunga, amasende, kanye ne-ovary.
Isu lokuhlanganisa iGenome de novo
Ci-anu (Ukulungisa) + WTDBG2 (Umhlangano) + Pilon(Polish) + Lachesis (Hi-C).
Imiphumela Eyinhloko
Umfanekiso 1. Izici ezibalulekile ze- common seadragon (P. taeniolatus) kanye ne-alligator pipefish (S. biaculeatus) kanye nokuma kwazo kwe-phylogenetic.
Umfanekiso 2. I-morphology kanye nokwakheka kofuzo kwama-appendages afana neqabunga kudragoni evamile yolwandle (P. taeniolatus).
Umfanekiso 3. Isakhi sofuzo esinqumayo sobulili kudragoni evamile (P. taeniolatus) kanye ne-alligator pipefish (S. biaculeatus).
Umfanekiso 4. I-Pharyngeal tooth phenotypes ku-zebrafish scpp5 i-homozygous mutants.
Isikhathi sokuthumela: Jan-08-2022