Alaabta

Taxanaha DNA/RNA - Taxanaha Nanopore

Taxanaha ONT waa hal molecule-waqtiga dhabta ah ee tignoolajiyada isku xigxiga signalka korantada ee ku salaysan nanopores, mabda'a isku xigxiga ee madal kasta waa isku mid.DNA/RNA laba-jibbaaran waxay ku xidhi doontaa borotiinka nanoporous ee ku dhex jira biofilm oo ka soo baxaya hogaanka borotiinka mootada, iyadoo la raacayo ficilka farqiga korantada ee labada dhinac ee biofilm, xargaha DNA / RNA waxay dhex maraan borotiinka kanaalka nanopore waqti cayiman. heerka.Sababtoo ah kala duwanaanshaha sifooyinka kiimikada ee saldhigyada kala duwan ee DNA / RNA, marka hal saldhig ama DNA molecule uu dhex maro kanaalka nanopore, waxay keeni doontaa isbeddelka calaamadaha korantada ee kala duwan.Iyadoo la ogaanayo oo u dhiganta calaamadahan, noocyada asaasiga ah ee u dhigma waa la xisaabin karaa, iyo ogaanshaha waqtiga dhabta ah ee isku xigxiga waa la dhammeyn karaa.

Nala soo xiriir

Faahfaahinta Adeegga

Natiijada Demo

Tilmaamaha Faahfaahinta Adeegga

Platform	Cabbirka Maktabadda	Wax-soo-saarka Xogta Aragtida (Qofkii unug)	Saxnaanta hal-saldhigga	Codsiyada
Nanopore	8Kb, 10kb, 20kb, Ultralong, cDNA-PCR	70-90Gb/Ceel	85-92%	Wicitaanka SV, De novo, Isku xigxiga dhererka buuxa, Iso-Seq, sharraxaadda hidda-wadaha, ogaanshaha methylation DNA

Faa'iidooyinka Adeegga

● In ka badan 5 sano oo waayo-aragnimo ah oo ku saabsan masraxa isku xigxiga ee PacBio oo leh kumanaan mashruuc oo xiran oo leh noocyo kala duwan.
● BMKGENE waa lammaane rasmi ah oo Oxford Nanopore ah, oo leh shahaadada RNA/DNA ee dual platform ah.
● Waxa jira moodooyin taxane ah oo isku xiga oo leh qalab dhamaystiran iyo soo gudbin ku filan oo taxane ah.
● Iyada oo ku saleysan goobta Nanopore, in ka badan 10 xayawaan iyo cilmi-baaris Denovo dhirta ayaa lagu daabacay joornaalada caanka ah ee caalamiga ah.

Shuruudaha Tusaalaha

Tusaalaha Nooca

Qadarka

Isku-dubarid (Qubit ®)

Mugga

daahirnimo

Kuwo kale

DNA-da genomiga ah

Waxay kuxirantahay Shuruudaha Xogta

≥20ng/μl

≥15ml

OD260/280=1.7-2.2;

OD260/230≥1.5;

Nadiifi meesha ugu sarreysa 260 nm, ma jiraan wasakh

Xoog saarista waxa loo baahan yahay in lagu cabbiro Qubit iyo Qubit/Nanopore ≤ 2

Wadarta RNA

≥1.2μg

≥100μg/μl

≥15ml

OD260/280=1.7-2.5;

OD260/230=0.5-2.5; wasakhaysan

Qiimaha RIN ≥7.5

Socodka Shaqada Adeegga

Tusaalaha diyaarinta

Dhismaha maktabadda

Isku xigxiga

Falanqaynta xogta

gaarsiinta mashruuca

Hore: Taxanaha DNA/RNA – Taxanaha Illumina

Xiga: Taxanaha Jajabka La Weyneeyay ee Gaarka ah-Locus (SLAF-Seq)

Qiimaynta Tayada Xogta ee Tusaalaha DNA

Shaxda 1. Tirakoobka xogta nadiifka ah.

BMKID	rawSeqNum	rawSumBase	cleanSeqNum	cleanSumBase	nadiif ahN50Len	nadiif ahN90Len	cleanMeanLen	cleanMaxLen	cleanMeanQual
DNA_BMK01	1,218,239	26.37	1,121,736	25.90	28,014	15,764	23,090	143,181	9