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Chromatin Immunoprecipitation Sequencing (ChIP-seq)
ChIP-Seq imapereka mbiri ya genome-wide ya DNA zomwe zikufuna kusintha histone, transcript factor, ndi mapuloteni ena okhudzana ndi DNA.Imaphatikiza kusankha kwa chromatin immuno-precipitation (ChIP) kuti ipezenso ma protein-DNA complexes, ndi mphamvu ya m'badwo wotsatira (NGS) pakutsatizana kwapamwamba kwa DNA yobwezeretsedwa.Kuphatikiza apo, chifukwa ma protein-DNA complexes amatengedwa kuchokera ku maselo amoyo, malo omangira amatha kufananizidwa mumitundu yosiyanasiyana ya maselo ndi minofu, kapena pansi pamikhalidwe yosiyana.Mapulogalamuwa amachokera ku malamulo olembedwa mpaka njira zachitukuko kupita ku njira za matenda ndi kupitirira.
Pulatifomu: Illumina NovaSeq Platform
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Metagenomic Sequencing -NGS
Metagenome imatanthawuza kusonkhanitsa kwa chibadwa chamagulu osakanikirana a zamoyo monga chilengedwe, metagenome yaumunthu, ndi zina zotero. Lili ndi majeremusi a tizilombo tomwe timalima komanso tosalimidwa.Metagenomic sequencing ndi chida cha mamolekyu chomwe chimagwiritsidwa ntchito posanthula zinthu zosakanizika za ma genomic zotengedwa ku zitsanzo zachilengedwe, zomwe zimapereka chidziwitso chambiri pamitundu yosiyanasiyana komanso kuchuluka kwake, kuchuluka kwa anthu, ubale wa phylogenetic, majini ogwira ntchito komanso maukonde olumikizana ndi chilengedwe.
nsanja:Illumina NovaSeq Platform
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Metagenomic Sequencing-Nanopore
Metagenomics ndi chida cha molekyulu chomwe chimagwiritsidwa ntchito pofufuza zinthu zosakanikirana zamtundu wamtundu zomwe zimatengedwa kuchokera ku zitsanzo zachilengedwe, zomwe zimapereka chidziwitso chambiri pamitundu yosiyanasiyana ya zamoyo ndi kuchuluka kwake, kuchuluka kwa anthu, ubale wa phylogenetic, majini ogwirira ntchito ndi maukonde ogwirizana ndi zinthu zachilengedwe, etc. Mapulatifomu a Nanopore adayambitsa posachedwa ku maphunziro a metagenomic.Kuchita kwake kwabwino pakuwerenga kwanthawi yayitali kunapititsa patsogolo kusanthula kwa metagenomic, makamaka msonkhano wa metagenome.Kutengera ubwino wowerengera kutalika, kafukufuku wa Nanopore-based metagenomic amatha kukwaniritsa msonkhano wopitilirabe poyerekeza ndi kuwombera mfuti.Zasindikizidwa kuti metagenomics yochokera ku Nanopore idapanga bwino ma genome athunthu komanso otsekedwa a bakiteriya kuchokera ku ma microbiomes (Moss, EL, et. al,Nature Biotech, 2020)
nsanja:Nanopore PromethION P48
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Kutsatizana konse kwa genome bisulfite
DNA methylation pamalo achisanu mu cytosine (5-mC) imakhudza kwambiri mafotokozedwe a majini ndi zochitika zama cell.Ma methylation osadziwika bwino amalumikizidwa ndi mikhalidwe ndi matenda angapo, monga khansa.WGBS yakhala muyezo wagolide wophunzirira genome-wide methylation pa single base resolution.
Pulatifomu: Illumina NovaSeq Platform
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Kuyesa kwa Chromatin Yopezeka ndi Transposase yokhala ndi Sequencing High Throughput (ATAC-seq)
ATAC-seq ndi njira yotsatirira kwambiri yowunikira kupezeka kwa chromatin ya genome, yomwe ndiyofunikira pakuwongolera ma epigenetic padziko lonse lapansi pakuwonetsa ma jini.Ma adapter otsatizana amalowetsedwa m'magawo otseguka a chromatin ndi hyperactive Tn5 transposase.Pambuyo pakukulitsa kwa PCR, laibulale yotsatizana imapangidwa.Magawo onse a chromatin otseguka amatha kupezeka pansi pa nthawi yeniyeni ya nthawi, osati kungokhala ndi malo omangirira a chinthu cholembera, kapena dera linalake losinthidwa la histone.
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16S/18S/ITS Amplicon Sequencing-PacBio
Subunit pa 16S ndi 18S rRNA yomwe ili ndi zigawo zonse zotetezedwa kwambiri komanso zosinthika kwambiri ndi chala chabwino kwambiri cha molekyulu yozindikiritsa zamoyo za prokaryotic ndi eukaryotic.Kutengera mwayi wotsatizana, ma amplicon awa amatha kuyang'ana pazigawo zosungidwa ndipo madera osinthika amatha kudziwika bwino kuti tizindikire tizilombo toyambitsa matenda zomwe zimathandizira pamaphunziro okhudza kusanthula kwamitundu yosiyanasiyana, taxonomy, phylogeny, ndi zina. ) kutsatizana kwa nsanja ya PacBio kumathandizira kupeza zowerengera zolondola kwambiri, zomwe zimatha kuphimba ma amplicons aatali (pafupifupi 1.5 KB).Kuwona kokulirapo kwa ma genetic kumathandizira kwambiri kusintha kwa mitundu ya mabakiteriya kapena bowa.
nsanja:PacBio Sequel II
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16S/18S/ITS Amplicon Sequencing-NGS
16S/18S/ITS amplicon sequencing ikufuna kuwulula kuchuluka kwa phylogeny, taxonomy, ndi mitundu yamitundu m'magulu ang'onoang'ono pofufuza zinthu za PCR za zolembera zanyumba zomwe zili ndi magawo omwe amakambirana kwambiri komanso osasinthika.Kuyambitsidwa kwa zala zabwino kwambiri zamamolekyulu olembedwa ndi Woeses et al, (1977) kumapereka mphamvu zodzipatula za microbiome.Kutsatizana kwa 16S (mabakiteriya), 18S (bowa) ndi Internal transcribed spacer(ITS, bowa) amalola kuzindikirika kwa mitundu yambirimbiri komanso mitundu yosowa komanso yosadziwika.Tekinoloje iyi yakhala chida chogwiritsidwa ntchito kwambiri komanso chachikulu pakuzindikiritsa mitundu yosiyanasiyana ya tizilombo m'malo osiyanasiyana, monga pakamwa pamunthu, matumbo, ndowe, ndi zina zambiri.
nsanja:Illumina NovaSeq Platform
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Mabakiteriya ndi fungal Yense ya Genome Yotsatizana
Kutsatizananso kwa ma genome ndi mabakiteriya ndi mafangasi ndi chida chofunikira kwambiri pomaliza ma genome a mabakiteriya odziwika ndi mafangasi, komanso kuyerekeza ma genome angapo kapena kujambula ma genome a zamoyo zatsopano.Ndikofunikira kwambiri kutsata ma genome athunthu a bakiteriya ndi bowa kuti apange ma genomes olondola, kupanga zozindikiritsa tizilombo ndi maphunziro ena ofananiza ma genome.
Pulatifomu: Illumina NovaSeq Platform
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PacBio-Full-length 16S/18S/ITS Amplicon Sequencing
Pulatifomu ya Amplicon (16S/18S/ITS) imapangidwa ndi zaka zambiri zakusanthula kwama projekiti osiyanasiyana, omwe amakhala ndi kusanthula koyambira komanso kusanthula kwamunthu payekha: kusanthula kofunikira kumakhudza kusanthula kwakukulu kwa kafukufuku waposachedwa wa tizilombo tating'onoting'ono, zowunikira ndizolemera komanso zomveka, ndi zotsatira za kusanthula zimaperekedwa mu mawonekedwe a malipoti a polojekiti;Zomwe zili pakuwunika kwamunthu ndizosiyanasiyana.Zitsanzo zitha kusankhidwa ndipo magawo atha kukhazikitsidwa mosinthika malinga ndi lipoti lowunikira komanso cholinga cha kafukufuku, kuti mukwaniritse zomwe mukufuna.Mawindo opangira makina, osavuta komanso ofulumira.
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PacBio-Full-length Transcriptome (Non-Reference)
Kutenga data ya Pacific Bioscience (PacBio) Isoform yotsatirira monga zolowetsa, Pulogalamuyi imatha kuzindikira masanjidwe aatali athunthu (popanda kuphatikiza).Popanga mapu a mautali athunthu motsutsana ndi ma genome, zolembedwa zimatha kukongoletsedwa ndi majini odziwika, zolembedwa, zigawo zolembera, ndi zina zotere. Pankhaniyi, kuzindikirika kolondola kwa mapangidwe a mRNA, monga kuphatikizika kwina, ndi zina, zitha kukwaniritsidwa.Kusanthula kophatikizana ndi data ya NGS transcriptome sequencing kumathandizira kutanthauzira momveka bwino komanso kuchulukitsidwa kolondola kwambiri pofotokozera pamlingo wa transcript, zomwe zimapindulitsa kwambiri kufotokozera kosiyanasiyana komanso kusanthula magwiridwe antchito.
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Kuchepetsa Kuyimilira kwa Bisulfite Sequencing (RRBS)
Kafukufuku wa DNA methylation nthawi zonse wakhala mutu wovuta kwambiri pa kafukufuku wa matenda, ndipo umagwirizana kwambiri ndi maonekedwe a jini ndi makhalidwe a pheno-typic.RRBS ndi njira yolondola, yothandiza komanso yachuma pa kafukufuku wa DNA methylation.Kulemeretsa kwa zigawo za zilumba za CpG ndi enzymatic cleavage (Msp I), kuphatikizidwa ndi kutsatizana kwa Bisulfite, kumapereka kuzindikira kwakukulu kwa DNA methylation.
Pulatifomu: Illumina NovaSeq Platform
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Kutsata kwa Prokaryotic mRNA
Kutsatizana kwa mRNA kumapatsa mphamvu kusanthula kwatsatanetsatane kwa zolembedwa zonse za mRNA m'maselo malinga ndi momwe zinthu ziliri.Ukadaulo wotsogolawu umagwira ntchito ngati chida champhamvu, kuwulula mbiri yakale ya jini, kapangidwe ka majini, ndi njira zama cell zomwe zimagwirizanitsidwa ndi mitundu yosiyanasiyana yachilengedwe.Kutengera kwambiri pakufufuza kofunikira, zowunikira zamankhwala, komanso kakulidwe ka mankhwala, kutsatizana kwa mRNA kumapereka chidziwitso pazovuta zama cellular dynamics ndi genetic regulation.Kukonzekera kwathu kwachitsanzo kwa prokaryotic mRNA kumapangidwira zolemba za prokaryotic, zomwe zikuphatikiza kuchepa kwa rRNA komanso kukonzekera kwama library.
Platform: Illumina NovaSeq X
