lncRNA
ʻO nā RNA lōʻihi ʻaʻole coding (lncRNA) he mau RNA ʻoi aku ka lōʻihi ma mua o 200 mau nucleotides me ka hiki ke coding haʻahaʻa akā me nā hana hoʻoponopono koʻikoʻi. Ua hoʻolālā ʻia ka pipeline BMKCloud lncRNA e kālailai i nā waihona puke rRNA i hoʻopau ʻia me kahi genome kuhikuhi kiʻekiʻe, i hoʻopaʻa maikaʻi ʻia, ma ka nānā ʻana i ka lncRNA a me ka hōʻike ʻana o ka mRNA. Ma hope o ka heluhelu ʻana i ka ʻoki ʻana a me ka kaohi ʻana i ka maikaʻi, ua hoʻohālikelike ʻia nā heluhelu i ka genome kuhikuhi e hōʻuluʻulu i nā transcripts, a ʻo ka loiloi ʻano gene ma hope e hōʻike ana i ka splicing ʻē aʻe a me nā genes hou. Ua ʻike ʻia nā transcripts he mRNAs a i ʻole lncRNAs, a ʻike ka loiloi hōʻike ʻokoʻa i nā lncRNA i hōʻike ʻokoʻa ʻia, kā lākou mau pahuhopu a me nā genes i hōʻike ʻokoʻa ʻia (DEGS). Ua hoʻopaʻa ʻia nā DEG a me nā pahuhopu lncRNA i hōʻike ʻokoʻa ʻia e ʻike i nā māhele hana i hoʻonui ʻia.
ʻIkepili Olaola
