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Metagenomic Sequencing (NGS)
Metagenome e bolela pokello ea kakaretso ea liphatsa tsa lefutso tsa sehlopha se tsoakaneng sa lintho tse phelang, tse kang metagenome ea tikoloho, metagenome ea batho, joalo-joalo. E na le liphatsa tsa lefutso tsa likokoana-hloko tse lengoang le tse ke keng tsa lengoa.Metagenomic sequencing ke sesebelisoa sa limolek'hule se sebelisetsoang ho hlahloba lisebelisoa tse tsoakiloeng tsa genomic tse ntšitsoeng lisampoleng tsa tikoloho, tse fanang ka boitsebiso bo qaqileng ka mefuta-futa ea mefuta le bongata, sebopeho sa baahi, kamano ea phylogenetic, liphatsa tsa lefutso tse sebetsang le marang-rang a amanang le tikoloho.
Sethala:Illumina NovaSeq6000
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Metagenomic Sequencing-Nanopore
Metagenomics ke sesebelisoa sa molek'hule se sebelisetsoang ho hlahloba lisebelisoa tse tsoakiloeng tsa genomic tse ntšitsoeng lisampoleng tsa tikoloho, tse fanang ka boitsebiso bo qaqileng ka mefuta-futa ea mefuta le bongata, sebopeho sa baahi, kamano ea phylogenetic, liphatsa tsa lefutso tse sebetsang le marang-rang a amanang le maemo a tikoloho, joalo-joalo Li-platform tsa ho latellana tsa Nanopore li sa tsoa hlahisoa. ho ea lithutong tsa metagenomic.Ts'ebetso ea eona e ikhethang ea bolelele ba ho bala e ntlafalitse haholo tlhahlobo ea metagenomic, haholo kopano ea metagenome.Ho nka melemo ea bolelele ba ho bala, boithuto ba metagenomic bo thehiloeng ho Nanopore bo khona ho fihlela kopano e tsoelang pele ha e bapisoa le metagenomics ea sethunya.Ho phatlalalitsoe hore metagenomics e thehiloeng ho Nanopore e atlehile ho hlahisa liphatsa tsa lefutso tsa baktheria tse felletseng le tse koetsoeng ho tsoa ho li-microbiomes (Moss, EL, et. al,Nature Biotech, 2020)
Sethala:Nanopore PromethION P48
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16S/18S/ITS Amplicon Sequencing-PacBio
Subunit e ho 16S le 18S rRNA e nang le libaka tse bolokehileng haholo le tse feto-fetohang haholo ke monwana o phethahetseng oa limolek'hule bakeng sa boitsebahatso ba likokoana-hloko tsa prokaryotic le eukaryotic.Ho nka monyetla oa tatellano, li-amplicon tsena li ka shejoa ho ipapisitse le likarolo tse bolokiloeng mme libaka tse feto-fetohang li ka tsebahatsoa ka botlalo bakeng sa boitsebahatso ba likokoana-hloko tse kenyang letsoho liphuputsong tse mabapi le tlhahlobo ea mefuta-futa ea likokoana-hloko, taxonomy, phylogeny, joalo-joalo. Molek'hule o le mong oa nako ea sebele(SMRT) ) tatellano ea sethala sa PacBio se nolofalletsa ho fumana lipalo tse telele tse nepahetseng haholo, tse ka koahelang li-amplicon tsa bolelele bo felletseng (hoo e ka bang 1.5 KB).Pono e pharalletseng ea lefapha la liphatsa tsa lefutso e matlafalitse haholo tharollo ea tlhaloso ea mefuta ea libaktheria kapa li-fungus.
Sethala:PacBio Sequel II
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16S/18S/ITS Amplicon Sequencing-NGS
Tatelano ea amplicon ea 16S/18S/ITS e ikemiselitse ho senola phylogeny, taxonomy, le mefuta e mengata sechabeng sa likokoana-hloko ka ho batlisisa lihlahisoa tsa PCR tsa liphatsa tsa lefutso tsa tlhokomelo ea ntlo tse nang le likarolo tse peli tse buuoang haholo le tse sa fetoheng.Kenyelletso ea menoana ena e phethahetseng ea limolek'hule ka Woeses et al, (1977) e matlafatsa profiling ea microbiome e se nang ho itšehla thajana.Tatellano ea 16S (libaktheria), 18S (fungus) le Internal transcribed spacer(ITS, fungus) e lumella ho tsebahatsa mefuta e mengata e mengata hammoho le mefuta e sa tloaelehang le e sa tsejoeng.Theknoloji ena e se e le sesebelisoa se sebelisoang haholo le se seholo ho khethollang sebopeho sa likokoana-hloko tse fapaneng libakeng tse fapaneng, joalo ka molomo oa motho, mala, mantle, joalo-joalo.
Sethala:Illumina NovaSeq6000
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Libaktheria le Fungus Whole Genome Re-sequencing
Tatelano e ncha ea genome ea baktheria le fungal ke sesebelisoa sa bohlokoa sa ho phethela liphatsa tsa lefutso tse tsejoang tsa baktheria le li-fungus, hammoho le ho bapisa liphatsa tsa lefutso tse ngata kapa ho etsa 'mapa oa liphatsa tsa lefutso tse ncha.Ho bohlokoa haholo ho tatellana liphatsa tsa lefutso tsa baktheria le li-fungus e le ho hlahisa liphatsa tsa lefutso tse nepahetseng, ho tsebahatsa likokoana-hloko le lithuto tse ling tsa papiso ea genome.
Sethala: Illumina NovaSeq 6000
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Genome ea fungal
Biomarker Technologies e fana ka tlhahlobo ea genome, genome e ntle le genome e felletseng ea fungal ho latela sepheo se ikhethileng sa lipatlisiso.Tatelano ea liphatsa tsa lefutso, kopano le tlhaloso e sebetsang e ka finyelloa ka ho kopanya tatellano ea moloko o latelang + Tatelano ea moloko oa boraro ho finyella kopano ea boemo bo phahameng ba genome.Theknoloji ea Hi-C e ka boela ea sebelisoa ho tsamaisa kopano ea genome boemong ba chromosome.
Sethala:PacBio Sequel II
Nanopore PromethION P48
Illumina NovaSeq 6000
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Libaktheria tse Feletseng Genome
Biomarker Technologies e fana ka ts'ebeletso ea tatellano mabapi le ho aha genome e felletseng ea libaktheria tse nang le lekhalo le zero.Ts'ebetso ea mantlha ea libaktheria kaho e felletseng ea liphatsa tsa lefutso e kenyelletsa tatellano ea moloko oa boraro, kopano, tlhaloso e sebetsang le tlhahlobo e tsoetseng pele ea bioinformatics e phethahatsang lipheo tse khethehileng tsa lipatlisiso.Profil e felletseng ea libaktheria tsa genome e fana ka matla a ho senola mekhoa ea mantlha ea ts'ebetso ea bona ea tlhaho, e ka fanang ka leseli la bohlokoa bakeng sa lipatlisiso tsa genomic mefuteng e phahameng ea eukaryotic.
Sethala:Nanopore PromethION P48 + Illumina NovaSeq 6000
PacBio Sequel II
