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Tsarin Metagenomic (NGS)
Metagenome yana nufin tarin jimillar kayan gado na gaurayawan al'umman halittu, kamar muhalli metagenome, metagenome na mutum, da sauransu. Ya ƙunshi kwayoyin halittar halittu masu rai da waɗanda ba za a iya nomawa ba.Tsarin metagenomic kayan aiki ne na kwayoyin halitta da ake amfani da su don nazarin gaurayen kayan halitta da aka samo daga samfuran muhalli, wanda ke ba da cikakkun bayanai game da bambancin nau'in da yawa, tsarin yawan jama'a, dangantakar halittu, kwayoyin halittar aiki da hanyar sadarwa tare da abubuwan muhalli.
Dandalin:Illumina NovaSeq6000
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Tsarin Metagenomic-Nanopore
Metagenomics shine kayan aikin kwayoyin halitta da aka yi amfani da su don daidaitattun kayan haɗin gwiwar da aka fitar, da kuma daidaitattun ilimin halittar jiki tare da dalilai na zamani, da sauransu na samar da dandamali na zamani, da sauransu. zuwa nazarin metagenomic.Fitaccen aikin sa a cikin tsayin karantawa ya inganta binciken metagenomic na rafi, musamman taron metagenome.Yin amfani da fa'idodin tsayin karantawa, nazarin metagenomic na tushen Nanopore yana iya samun ƙarin ci gaba da taro idan aka kwatanta da abubuwan metagenomics na harbi.An buga cewa Nanopore na tushen metagenomics sun sami nasarar haifar da cikakke kuma rufaffiyar kwayoyin halittar kwayoyin cuta daga microbiomes (Moss, EL, et. al,Nature Biotech, 2020)
Dandalin:Nanopore PromethION P48
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16S/18S/ITS Amplicon Sequencing-PacBio
Rukunin da ke kan 16S da 18S rRNA wanda ya ƙunshi yankuna biyun da aka kiyaye su sosai da kuma masu jujjuyawa shine cikakkiyar hoton yatsa na kwayoyin halitta don gano kwayoyin prokaryotic da eukaryotic.Shan amfani da sequencing, wadannan amplicons za a iya niyya bisa ga kiyaye sassa da hyper-canzawa yankuna za a iya cikakken hali ga microbial ganewa na ba da gudummawa ga binciken rufe microbial bambancin bincike, taxonomy, phylogeny, da dai sauransu Single-kwayoyin real-lokaci (SMRT). Tsarin tsarin dandamali na PacBio yana ba da damar samun ingantaccen dogon karantawa, wanda zai iya rufe amplicons masu tsayi (kimanin 1.5 Kb).Faɗin ra'ayi game da filin kwayoyin halitta ya inganta ƙudiri sosai a cikin bayanin nau'in ƙwayoyin cuta ko fungi.
Dandalin:PacBio Sequel II
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16S/18S/ITS Amplicon Sequencing-NGS
16S/18S/ITS amplicon sequencing da nufin bayyana phylogeny, haraji, da nau'i mai yawa a cikin al'ummar microbial ta hanyar binciken samfuran PCR na alamomin kwayoyin halitta waɗanda ke ƙunshe da ɓangarori biyu masu ma'ana da haɓakawa.Gabatar da waɗannan cikakkiyar sawun yatsa na ƙwayoyin cuta ta Woeses et al, (1977) yana ba da ikon keɓance bayanan microbiome mara amfani.Jeri na 16S (kwayoyin cuta), 18S (fungi) da na waje da aka rubuta na ciki (ITS, fungi) yana ba da damar gano nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan iri ne da ba a san su ba.Wannan fasaha ta zama kayan aiki da yawa da ake amfani da su kuma babban kayan aiki don gano nau'ikan ƙwayoyin cuta daban-daban a cikin yanayi daban-daban, kamar bakin ɗan adam, hanji, najasa, da sauransu.
Dandalin:Illumina NovaSeq6000
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Bacterial and Fungal Whole Genome Re-sequencing
Bacterial da fungal dukan kwayoyin halittar sake-sequencing kayan aiki ne mai mahimmanci don kammala kwayoyin halittu na ƙwayoyin cuta da fungi da aka sani, da kuma kwatanta kwayoyin halitta masu yawa ko yin taswirar kwayoyin halitta na sababbin kwayoyin halitta.Yana da matukar muhimmanci a jera dukkanin kwayoyin halittar kwayoyin cuta da fungi domin samar da ingantattun kwayoyin halittar kwayoyin halitta, don yin gano kwayoyin cuta da sauran nazarin kwayoyin halittar kwatankwacinsu.
Platform: Illumina NovaSeq 6000
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Fungal Genome
Fasahar Biomarker suna ba da binciken binciken kwayoyin halitta, kyawawan kwayoyin halitta da kuma cikakkiyar kwayar halittar fungi na pene dangane da takamaiman manufar bincike.Za'a iya samun tsarin tsarin halittar halitta, taro da bayanin aiki ta hanyar haɗa jerin abubuwan gaba-gaba + jerin tsararraki na uku don cimma babban taro na kwayoyin halitta.Hakanan ana iya amfani da fasahar Hi-C don sauƙaƙe haɗuwar kwayoyin halitta a matakin chromosome.
Dandalin:PacBio Sequel II
Nanopore PromethION P48
Illumina NovaSeq 6000
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Bacteria Complete Genome
Biomarker Technologies yana ba da sabis na jeri akan gina cikakkiyar kwayar halittar ƙwayoyin cuta tare da gibin sifili.Babban aikin ƙwayoyin cuta cikakken ginin kwayoyin halitta ya haɗa da jerin tsararrun tsararraki na uku, taro, bayanin aiki da ingantaccen bincike na bioinformatic wanda ke cika takamaiman manufofin bincike.Ƙarin cikakkun bayanan ƙwayoyin cuta na kwayoyin halitta yana ba da damar bayyana mahimman hanyoyin da ke ƙarƙashin tsarin rayuwarsu, wanda kuma zai iya ba da mahimmanci ga bincike na kwayoyin halitta a cikin nau'in eukaryotic mafi girma.
Dandalin:Nanopore PromethION P48 + Illumina NovaSeq 6000
PacBio Sequel II
