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ʻO Chromatin Immunoprecipitation Sequencing (ChIP-seq)
Hāʻawi ʻo ChIP-Seq i ka genome-wide profiling o nā pahuhopu DNA no ka hoʻololi ʻana i ka histone, nā kumu transcription, a me nā protein e pili ana i ka DNA.Hoʻohui ia i ke koho ʻana o ka chromatin immuno-precipitation (ChIP) e hoʻihoʻi i nā paʻakikī protein-DNA kikoʻī, me ka mana o ka sequencing hanauna hou (NGS) no ke kaʻina hana kiʻekiʻe o ka DNA i hoʻihoʻi ʻia.Hoʻohui ʻia, no ka loaʻa ʻana o nā paʻina protein-DNA mai nā sela ola, hiki ke hoʻohālikelike ʻia nā wahi paʻa i nā ʻano cell like ʻole a me nā ʻiʻo, a i ʻole ma lalo o nā kūlana like ʻole.Loaʻa nā noi mai ka hoʻoponopono transcriptional i nā ala hoʻomohala a hiki i nā mīkini maʻi a ma waho.
Papahana: Illumina NovaSeq 6000
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ʻO ke kaʻina o ka genome bisulfite holoʻokoʻa
ʻO ka methylation DNA ma ke kūlana ʻelima i ka cytosine (5-mC) he mana koʻikoʻi i ka hōʻike ʻana o ka gene a me ka hana kelepona.Ua hoʻopili ʻia nā ʻano methylation maʻamau me kekahi mau maʻi a me nā maʻi, e like me ke kanesa.Ua lilo ʻo WGBS i kumu gula no ke aʻo ʻana i ka genome-wide methylation ma ka hoʻonā kumu hoʻokahi.
Papahana: Illumina NovaSeq6000
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Ho'āʻo no ka Chromatin hiki ke hoʻololi ʻia me ke kaʻina hana kiʻekiʻe (ATAC-seq)
ʻO ATAC-seq kahi ala hoʻokaʻina kiʻekiʻe no ka nānā ʻana i ka hiki ʻana o ka genome-wide chromatin accessibility, he mea nui ia no ka mana epigenetic honua o ka hōʻike ʻana.Hoʻokomo ʻia nā mea hoʻopili sequencing i nā ʻāpana chromatin ākea e ka hyperactive Tn5 transposase.Ma hope o ka hoʻonui ʻia ʻana o PCR, kūkulu ʻia kahi waihona sequencing.Hiki ke loaʻa nā ʻāpana chromatin hāmama a pau ma lalo o kahi kūlana manawa-manawa kikoʻī, ʻaʻole i kaupalena wale ʻia i nā wahi paʻa o kahi mea unuhi, a i ʻole kekahi ʻāpana histone i hoʻololi ʻia.
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Hoʻemi ʻia ka hoʻohālikelike Bisulfite Sequencing (RRBS)
ʻO ka noiʻi DNA methylation he kumuhana wela i ka noiʻi maʻi, a pili pili i ka hōʻike gene a me nā ʻano pheno-typic.ʻO RRBS kahi ala pololei, kūpono a me ka waiwai no ka noiʻi DNA methylation.ʻO ka hoʻonui ʻia ʻana o ka mea hoʻolaha a me nā ʻāina mokupuni CpG e ka enzymatic cleavage (Msp I), i hui pū ʻia me ka Bisulfite sequencing, hāʻawi i ka ʻike kiʻekiʻe DNA methylation.
Papahana: Illumina NovaSeq 6000
