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Chromatin Immunoprecipitation Sequencing (ChIP-seq)
Chip-Seq muab genome-wide profileing ntawm DNA lub hom phiaj rau kev hloov kho histone, hloov pauv cov ntsiab lus, thiab lwm yam DNA-txuas nrog cov proteins.Nws muab cov kev xaiv ntawm chromatin immuno-precipitation (ChIP) kom rov qab tau cov protein-DNA complexes, nrog lub zog ntawm cov tiam tom ntej sequencing (NGS) rau high-throughput sequencing ntawm cov DNA zoo.Tsis tas li ntawd, vim hais tias cov protein-DNA complexes tau rov qab los ntawm cov hlwb nyob, cov chaw khi tuaj yeem muab piv rau ntau hom cell thiab cov ntaub so ntswg, lossis nyob rau hauv cov xwm txheej sib txawv.Cov ntawv thov sib txawv los ntawm kev tswj hwm kev hloov pauv mus rau txoj hauv kev loj hlob mus rau cov txheej txheem kab mob thiab dhau mus.
Platform: Illumina NovaSeq 6000
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Tag nrho genome bisulfite sequencing
DNA methylation ntawm txoj haujlwm thib tsib hauv cytosine (5-mC) muaj qhov cuam tshuam tseem ceeb ntawm cov noob qhia thiab kev ua haujlwm ntawm tes.Cov qauv methylation txawv txav tau cuam tshuam nrog ntau yam mob thiab kab mob, xws li mob qog noj ntshav.WGBS tau dhau los ua tus qauv kub rau kev kawm genome-wide methylation ntawm ib qho kev daws teeb meem.
Platform: Illumina NovaSeq6000
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Kev soj ntsuam rau Transposase-Accessible Chromatin nrog High Throughput Sequencing (ATAC-seq)
ATAC-seq yog ib txoj hauv kev zoo tshaj plaws rau kev soj ntsuam ntawm genome-wide chromatin accessibility, uas yog ib qho tseem ceeb rau ntiaj teb no epigenetic tswj ntawm noob qhia.Sequencing adapters yog muab tso rau hauv qhib chromatin cheeb tsam los ntawm hyperactive Tn5 transposase.Tom qab PCR amplification, lub tsev qiv ntawv sib txuas tau tsim.Tag nrho cov cheeb tsam qhib chromatin tuaj yeem tau txais nyob rau hauv ib qho chaw tshwj xeeb-lub sijhawm, tsis yog tsuas yog txwv rau cov chaw sib txuas ntawm qhov kev hloov pauv, lossis qee thaj tsam histone hloov kho.
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Txo Cov Neeg Sawv Cev Bisulfite Sequencing (RRBS)
Kev tshawb fawb DNA methylation yeej ib txwm yog lub ntsiab lus kub hauv kev tshawb fawb kab mob, thiab muaj feem cuam tshuam nrog cov noob qhia thiab pheno-typic zoo.RRBS yog ib txoj hauv kev zoo, muaj txiaj ntsig thiab kev lag luam rau kev tshawb fawb DNA methylation.Txhim khu kev txhawb nqa thiab CpG cov kob thaj tsam los ntawm enzymatic cleavage (Msp I), ua ke nrog Bisulfite sequencing, muab siab daws teeb meem DNA methylation nrhiav kom tau.
Platform: Illumina NovaSeq 6000
