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Tsarin mRNA mai cikakken tsayi-Nanopore
Jerin RNA ya kasance kayan aiki mai kima don cikakken bincike na kwafi.Babu shakka, jerin gajerun karatun gargajiya sun sami ci gaba mai yawa a nan.Duk da haka, sau da yawa yakan gamu da iyakancewa a cikin cikakkun bayanan isoform na tsawon tsayi, ƙididdigewa, son zuciya na PCR.
Tsarin Nanopore yana bambanta kansa da sauran dandamali na jeri, domin ana karanta nucleotide kai tsaye ba tare da haɗin DNA ba kuma yana haifar da dogon karantawa a dubun kilobases.Wannan yana ba da ikon ƙetare cikakken kwafin kwafin karatu kai tsaye da magance ƙalubalen a cikin karatun matakin isoform.
Dandalin:Nanopore PromethION
Laburare:cDNA-PCR
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De novo Cikakken Tsawon Rubuce-rubucen -PacBio
Da novojeri mai cikakken tsayin rubutu, wanda kuma aka sani daDa novoIso-Seq yana ɗaukar fa'idodin PacBio sequencer a cikin tsayin karantawa, wanda ke ba da damar jeri na cikakken tsayin ƙwayoyin cDNA ba tare da wani hutu ba.Wannan gaba ɗaya yana guje wa duk wani kurakurai da aka haifar a cikin matakan tattara bayanan rubutu kuma yana gina saiti na unigene tare da ƙudurin matakin isoform.Wannan saitin unigene yana ba da bayanan kwayoyin halitta masu ƙarfi a matsayin "kwayoyin halitta" a matakin rubutu.Bugu da ƙari, haɗawa tare da bayanan tsara tsararru na gaba, wannan sabis ɗin yana ba da ƙarfin ƙididdige ƙimar matakin isoform.
Platform: PacBio Sequel IILibrary: SMRT bell library -
Eukaryotic mRNA sequencing-Illumina
Tsarin mRNA yana ba da damar yin bayanin duk mRNAs da aka rubuta daga sel a ƙarƙashin takamaiman yanayi.Fasaha ce mai ƙarfi don bayyana bayanan bayanan kwayoyin halitta, tsarin kwayoyin halitta da hanyoyin kwayoyin wasu hanyoyin nazarin halittu.Har zuwa yau, an yi amfani da jeri na mRNA a cikin bincike na asali, bincike na asibiti, haɓaka magunguna, da sauransu.
Platform: Illumina NovaSeq 6000
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Tsarin mRNA wanda ba na Magana ba-Illumina
mRNA sequencing ya rungumi dabara na gaba-gaba (NGS) don kama manzo RNA(mRNA) ya samar da Eukaryote a takamaiman lokacin da wasu ayyuka na musamman ke kunnawa.Mafi tsayin rubutun da aka raba ana kiransa 'Unigene' kuma an yi amfani da shi azaman jeri na bincike na gaba, wanda shine ingantacciyar hanya don nazarin tsarin kwayoyin halitta da tsarin sadarwa na nau'in ba tare da tunani ba.
Bayan tattara bayanan kwafi da bayanin aikin unigene
(1) Binciken SNP, nazarin SSR, tsinkayar CDS da tsarin kwayoyin halitta za a riga an tsara su.
(2) Ƙididdigar maganganun unigene a cikin kowane samfurin za a yi.
(3)Unigenes daban-daban da aka bayyana tsakanin samfurori (ko ƙungiyoyi) za a gano su bisa lafazin unigene
(4) Za a yi tari, bayanin aiki da kuma ingantaccen bincike na unigenes da aka bayyana daban-daban.
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Dogon jerin abubuwan da ba a yi rikodin ba-Illumina
Dogayen RNAs marasa coding (lncRNAs) nau'in kwayoyin halittar RNA ne masu tsayin da ya wuce 200 nt, wadanda aka siffata su ta hanyar karancin coding.LncRNA, a matsayin maɓalli mai mahimmanci a cikin RNAs marasa coding, ana samun galibi a cikin tsakiya da plasma.Haɓakawa a cikin jerin abubuwan fasaha da bioinfortics yana ba da damar gano lncRNAs da yawa da kuma haɗa waɗanda ke da ayyukan ilimin halitta.Shaidu masu tarawa suna ba da shawarar cewa lncRNA yana da hannu sosai a cikin ƙa'idodin epigenetic, ƙa'idodin rubutawa da kuma ka'idojin rubutu bayan rubutu.
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Ƙananan jerin RNA-Illumina
Ƙananan RNA yana nufin nau'in kwayoyin RNA marasa coding waɗanda yawanci ba su wuce 200nt tsayi ba, gami da micro RNA (miRNA), ƙaramin tsangwama RNA (siRNA), da RNA (piRNA) mai hulɗa da piwi.
MicroRNA (miRNA) aji ne na ƙananan RNA mai ƙarfi tare da tsawon kusan 20-24nt, wanda ke taka muhimmiyar rawa na tsari iri-iri a cikin sel.miRNA ya shiga cikin matakai na rayuwa da yawa waɗanda ke bayyana nama - takamaiman da mataki - takamaiman magana kuma an kiyaye su sosai a cikin nau'ikan nau'ikan daban-daban.
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circRNA sequencing-Ilumina
An ƙera gabaɗayan jeri na kwafi don yin bayanin kowane nau'ikan kwayoyin RNA, gami da coding (mRNA) da RNAs marasa coding (ciki har da lncRNA, circRNA da miRNA) waɗanda takamaiman sel ke rubutawa a wani lokaci.Gabaɗayan jeri na rubutu, wanda kuma aka sani da “ jimlar jerin RNA” yana nufin bayyana cikakkun hanyoyin sadarwa na tsari a matakin rubutu.Yin amfani da fa'idar fasahar NGS, jerin samfuran samfuran kwafi duka suna samuwa don nazarin ceRNA da nazarin RNA na haɗin gwiwa, wanda ke ba da matakin farko zuwa halayen aiki.Bayyanar hanyar sadarwa ta hanyar ceRNA ta tushen circRNA-miRNA-mRNA.
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Cikakkun jerin bayanan da aka rubuta - Illumina
An ƙera gabaɗayan jeri na kwafi don yin bayanin kowane nau'ikan kwayoyin RNA, gami da coding (mRNA) da RNAs marasa coding (ciki har da lncRNA, circRNA da miRNA) waɗanda takamaiman sel ke rubutawa a wani lokaci.Gabaɗayan jeri na rubutu, wanda kuma aka sani da “ jimlar jerin RNA” yana nufin bayyana cikakkun hanyoyin sadarwa na tsari a matakin rubutu.Yin amfani da fa'idar fasahar NGS, jerin samfuran samfuran kwafi duka suna samuwa don nazarin ceRNA da nazarin RNA na haɗin gwiwa, wanda ke ba da matakin farko zuwa halayen aiki.Bayyanar hanyar sadarwa ta hanyar ceRNA ta tushen circRNA-miRNA-mRNA.
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Tsarin RNA Prokaryotic
Sequencing na Prokaryotic RNA yana amfani da jerin tsararraki na gaba (NGS) don bayyana kasancewar da adadin RNA a wani ɗan lokaci, ta hanyar nazarin canjin salon salula.Sequencing na kamfaninmu na prokaryotic RNA, musamman yana nufin prokaryotes tare da nau'ikan kwayoyin halitta, yana ba ku bayanan bayanan rubutu, nazarin tsarin kwayoyin halitta, da sauransu. An yi amfani da shi sosai ga binciken kimiyya na asali, binciken magunguna da haɓakawa, da ƙari.
Platform: Illumina NovaSeq 6000
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Metatranscriptome Sequencing
Metatranscriptome sequencing yana nuna bayanan kwayoyin halitta na microbes (duka eukaryotes da prokaryotes) a cikin mahalli na halitta (watau ƙasa, ruwa, teku, najasa, da gut). Naƙiƙa, nazarin ƙirƙirar halittu daban-daban da aka bayyana halittu, da ƙari.
Platform: Illumina NovaSeq 6000
