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Chirimwa/Mhuka De novo Genome Sequencing
De novokutevedzana kunoreva kuvakwa kwejenome rerudzi rwemhando yose vachishandisa matekinoroji ekutevedzanisa, semuenzaniso PacBio, Nanopore, NGS, zvichingodaro, pasina genome rereferensi.Kuvandudzwa kunoshamisa mukuverenga kureba kwechizvarwa chechitatu kutevedzana matekinoroji kwakaunza mikana mitsva mukuunganidza majenomes akaoma, akadai seaya ane heterozygosity yakakura, chiyero chepamusoro chenzvimbo dzinodzokororwa, mapolyploid, nezvimwewo. kugadzirisa zvinhu zvinodzokororwa, matunhu ane abnormal GC zviri mukati uye mamwe matunhu akaomarara.
Platform: PacBio Sequel II / Nanopore PromethION P48/ Illumina NovaSeq6000
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Hi-C yakavakirwa Genome Assembly
Hi-C inzira yakagadzirirwa kubata chromosome kumisikidzwa nekubatanidza kuongorora kudhuze-kwakavakirwa kupindirana uye yakakwirira-kuburikidza kutevedzana.Kusimba kwekudyidzana uku kunofungidzirwa kuve kwakabatana zvisina kunaka nehuremu hwemuviri pamakromosomes.Naizvozvo, Hi-C data inogona kutungamira kuunganidzwa, kurongeka uye kutungamira kweakaunganidzwa akateedzana mugwaro genome uye nekumisa iwo pane imwe nhamba yemakromosomes.Iyi tekinoroji inopa simba kugungano re chromosome-level genome pasina huwandu hwevanhu-based genetic mepu.Yese genome imwe inoda Hi-C.
Platform: Illumina NovaSeq6000 / DNBSEQ
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Evolutionary Genetics
Evolutionary genetics ibasa rakazara rekutevedzana rakagadzirirwa kupa dudziro yakazara paruzivo rwekushanduka kwezvinhu zvakapihwa zvichienderana nekusiyana kwemajini, kusanganisira SNPs, InDels, SVs uye CNVs.Inopa kuongorora kwese kwakakosha kunodiwa pakutsanangura shanduko yekushanduka-shanduka uye maumbirwo emarudzi evanhu, zvakadai sehuwandu hwevanhu, kusiyana kwemajini, hukama hwefilogeny, nezvimwewo. Iinewo zvidzidzo pamusoro pekuyerera kwemajini, izvo zvinopa simba kufungidzira kwehuwandu hwevanhu hunobudirira, nguva yekusiyana.
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Kuenzanisa Genomics
Comparative genomics zvinoreva kuenzanisa kutevedzana kwegenome uye zvimiro zvemarudzi akasiyana.Ichi chirango chine chinangwa chekuburitsa kushanduka kwemhando, basa remajini, maitiro ekudzora majini padanho rejenome nekuona kutevedzana zvimiro uye zvinhu zvakachengetedza kana kupatsanura mumarudzi akasiyana.Yakajairika yekuenzanisa genomics kudzidza inosanganisira ongororo mumhuri yejini, kusimukira kwekushanduka, kudzokorodza kwegenome yakazara, kudzvanywa kwekusarudza, nezvimwe.
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Bulked Segregant analysis
Bulked segregant analysis (BSA) inzira inoshandiswa kukurumidza kuona phenotype yakabatana genetic markers.Main workflow yeBSA ine kusarudza mapoka maviri evanhu vane phenotypes anopokana zvakanyanya, kubatanidza DNA yevanhu vese kuti vaumbe maviri akawanda eDNA, inozivisa mutsauko wakatevedzana pakati pemadziva maviri.Iyi tekinoroji yakashandiswa zvakanyanya mukuziva ma genetic mamaki akabatana zvakanyanya neanonangwa majini muchirimwa / mhuka genomes.
