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Zomera / Zinyama za De novo Genome Sequencing
Pa novokutsatizana kumatanthauza kupanga ma genome amtundu wonse pogwiritsa ntchito umisiri wotsatizana, mwachitsanzo, PacBio, Nanopore, NGS, ndi zina zotere, ngati palibe jini lolozera.Kuwongolera kodabwitsa pakuwerenga kwautali waukadaulo wotsatizana wa m'badwo wachitatu kwabweretsa mwayi watsopano pakusonkhanitsa ma genome ovuta, monga omwe ali ndi heterozygosity yayikulu, chiŵerengero chambiri cha zigawo zobwerezabwereza, ma polyploids, ndi zina zambiri. Kuthetsa zinthu zobwerezabwereza, madera omwe ali ndi GC zachilendo ndi zigawo zina zovuta kwambiri.
Platform: PacBio Sequel II / Nanopore PromethION P48/ Illumina NovaSeq6000
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Hi-C yochokera ku Genome Assembly
Hi-C ndi njira yomwe idapangidwa kuti ijambule masinthidwe a chromosome pophatikiza kuyesa kuyanjana kotsatana ndi kuyandikana komanso kutsata kwapamwamba.Kuchuluka kwa kuyanjana uku kumakhulupirira kuti kumalumikizidwa molakwika ndi mtunda wapakatikati pa ma chromosome.Chifukwa chake, data ya Hi-C imatha kutsogolera kusanjikizana, kuyitanitsa ndi kuwongolera katsatidwe kamene kamasonkhanitsidwa mumtundu wa genome ndikumangirira pamitundu ina ya ma chromosome.Ukadaulo uwu umathandizira kuphatikiza kwa ma chromosome genome popanda mapu otengera kuchuluka kwa anthu.Genome iliyonse imafunikira Hi-C.
Platform: Illumina NovaSeq6000 / DNBSEQ
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Evolutionary Genetics
Evolutionary genetics ndi ntchito yotsatizana yomwe idapangidwa kuti ipereke kutanthauzira kwatsatanetsatane pazachisinthiko zazinthu zoperekedwa motengera kusiyanasiyana kwa majini, kuphatikiza ma SNP, InDels, SVs ndi CNVs.Limapereka zowunikira zonse zofunika pofotokoza za kusintha kwa chisinthiko ndi ma genetic a anthu, monga kuchuluka kwa anthu, kusiyanasiyana kwa ma genetic, maubale a phylogeny, ndi zina zambiri. Lilinso ndi maphunziro okhudza kutuluka kwa majini, komwe kumathandizira kuyerekezera kuchuluka kwa anthu, nthawi yakusiyana.
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Kufananiza Genomics
Comparative genomics kwenikweni amatanthauza kufanizitsa ndondomeko yonse ya ma genome ndi mapangidwe a mitundu yosiyanasiyana.Lamuloli likufuna kuwulula zakusintha kwa mitundu, magwiridwe antchito a majini, njira zowongolera ma jini pamlingo wa genome pozindikira momwe amayenderana ndi zinthu zomwe zimasunga kapena kusiyanitsa mitundu yosiyanasiyana yamitundu.Kafukufuku wofananira wa ma genomics amaphatikizanso kusanthula m'mabanja a majini, kakulidwe kachisinthiko, kubwerezabwereza kwamtundu wonse, kukakamiza kosankha, ndi zina zambiri.
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Bulked Segregant kusanthula
Bulked segregant analysis (BSA) ndi njira yomwe imagwiritsidwa ntchito pozindikira mwachangu zolembera zamtundu wa phenotype.Kuyenda kwakukulu kwa BSA kumakhala ndi kusankha magulu awiri a anthu omwe ali ndi ma phenotypes otsutsana kwambiri, kuphatikiza DNA ya anthu onse kuti apange ma DNA awiri, ndikuzindikira kutsatizana kosiyana pakati pa maiwe awiri.Njira imeneyi yagwiritsidwa ntchito kwambiri pozindikiritsa zolembera za majini zomwe zimagwirizanitsidwa kwambiri ndi majini omwe amawunikira mumagulu a zomera/zinyama.
