Ngwaahịa

Usoro DNA/RNA - usoro Nanopore

Usoro DNA/RNA – Ihe onyonyo nke Nanopore Sequencer

Usoro ONT bụ otu molekul ezigbo oge mgbaama eletriki usoro teknụzụ dabere na nanopores, ụkpụrụ usoro nke ikpo okwu ọ bụla bụ otu.DNA/RNA nwere eriri abụọ ga-ejikọta na protein nanoporous agbakwunyere na biofilm ma na-apụ apụ n'okpuru nduzi nke protein moto, n'okpuru ọrụ nke voltaji dị iche na akụkụ abụọ nke biofilm, DNA / RNA strands na-agafe na protein channel nanopore na ụfọdụ. ọnụego.N'ihi ọdịiche dị n'ụdị kemịkalụ nke ntọala dị iche iche na eriri DNA / RNA, mgbe otu isi ma ọ bụ DNA molecule gafere na ọwa nanopore, ọ ga-eme ka mgbanwe nke akara eletrik dị iche iche.Site n'ịchọpụta na kwekọrọ na akara ndị a, enwere ike ịgbakọ ụdị ntọala kwekọrọ na ya, na nchọpụta oge nke usoro nwere ike mechaa.

Kpọtụrụ anyị

Nkọwa ọrụ

Nsonaazụ ngosi

Atụmatụ nkọwa ọrụ

Platform	Nha ọba akwụkwọ	Mpụta data usoro ihe omume (kwa otu cell)	Izizi otu ntọala	Ngwa
Nanopore	8Kb, 10kb, 20kb, Ultralong, cDNA-PCR	70-90Gb/Sell	85-92%	Ịkpọ oku SV, De novo, Usoro ogologo zuru oke, Iso-Seq, nkọwa mkpụrụ ndụ ihe nketa, Nchọpụta DNA methylation

Uru ọrụ

● Ahụmahụ ihe karịrị afọ 5 na usoro usoro PacBio nwere ọtụtụ puku ọrụ mechiri emechi nwere ụdị dị iche iche.
● BMKGENE bụ onye ọrụ gọọmentị Oxford Nanopore, nwere asambodo RNA/DNA abụọ.
● E nwere ụdị usoro ihe eji eme ihe na akụrụngwa zuru oke yana ntinye usoro zuru oke.
● Dabere na ikpo okwu Nanopore, ebipụtala ihe karịrị 10 anụmanụ na osisi Denovo nyocha n'akwụkwọ akụkọ ndị a ma ama n'ụwa niile.

Ihe atụ chọrọ

Ụdị Nlereanya

Ọnụ ego

Ntụnye (Qubit ®)

Olu

Ịdị ọcha

Ndị ọzọ

Genomic DNA

Dabere na data chọrọ

≥20ng/μl

≥15 ml

OD260/280=1.7-2.2;

OD260/230≥1.5;

Kpochapụ elu na 260 nm, enweghị mmetọ

Ekwesịrị iji Qubit na Qubit/Nanopore tụọ ntinye uche ≤ 2

Mkpokọta RNA

≥1.2μg

≥100μg/μl

≥15 ml

OD260/280=1.7-2.5;

OD260/230 = 0.5-2.5; enweghị mmetọ

Ọnụ ego RIN ≥7.5

Usoro ọrụ ọrụ

Nkwadebe ihe atụ

Ịrụ ụlọ akwụkwọ

Usoro

Nyocha data

Nnyefe oru ngo

Nke gara aga: Usoro DNA/RNA - Ihe na-eme Illumina

Osote: Usoro Mpempe Mpempe Akọwapụtara kpọmkwem-Locus (SLAF-Seq)

Ntụle ogo data nke nlele DNA

Tebụl 1. Ọnụ ọgụgụ na data dị ọcha.

BMKID	rawSeqNum	rawSumBase	CleanSeqNum	CleanSumBase	CleanN50 Len	CleanN90Len	CleanMeanLen	CleanMaxLen	CleanMeanQual
DNA_BMK01	1,218,239	26.37	1,121,736	25.90	28,014	15,764	23,090	143,181	9