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Single- nuclei RNA sequencing

Et Development of Una-cell captis et consuetudo Library constructione artes, copulata cum summus throughput sequencing, habet revolutioned gene expressio studiis in cellula gradu. Hoc breakthrough concedit pro altius et magis comprehensive analysis of complexu cellula populos, vincat limitations consociata cum averaging gene expressio super omnes cellulis et conservando verum heterogeneum in his populationibus. Dum unum-cell rna sequencing (Scrorum, seq) habet undeniable commoda, id certamicis provocationes in certa textuum ubi creatio de uno-cell suspensionem probat difficile et requirit nova exempla. In BMKNGGGene, nos address this HRURDLE per offering Una-nucleus RNA sequencing (SNRNA, seq) per statum-of-in-es 10x genomics Chromium technology. Hoc approach latior spectro ex samples amenable ad transcriptome analysis in unum cell campester.

Solante nuclei fit per innovative 10x genomics Chromium chip, featuring an octo-channel microfluidics ratio in duplici transitus. In hac ratio, gel Barcodes Barcodes Barcodes Barcodes Barcodes, Enzymes et unum nucleum in encapsulated in Nanoliter-amplitudo oleum guttae, formando gel bead-in EMULSIO (gem). Post gemma formation, cellula lysis et barcode release occurrunt intra se gemma. Deinde, mRNA Molecules subeunt transcribenda in CDNAS, incorporating 10x Barcodes et unique Molecular identifiers (Umis). Haec CDNAS sunt igitur subiecta ad vexillum sequencing bibliotheca constructione, facilatio robust et comprehensive exploratio de gene expressio profiles in unum cell campester.

Platform: X × genomics Chromium et Illumina Novaseq Platform

Service Details

Bioinformatics

Demo Results

Featured Publications

Technical machinemur

Solante nuclei est effectum per X × genomics Chromium ™, quod consistit octo-channel microfluidics ratio cum duplici transitus. In hoc ratio, a gel barcodes et primario, enzymes et una nucleus sunt encapsulated in nanoliter-amplitudo oleum gutta, generating gel bead-in EMULSIO (gem). Cum gemma formatae sunt, cellula lysis et dimittis de barcodes fiunt in se gemma. MRNA sunt e converso transcribed in CDNA moleculis cum X × barcodes et UMI, quae sunt ulterius subiecti ad vexillum sequencing bibliothecam constructione.

Features

● Praeparatio unius-nuclei suspensionem fringitur fibras

● formatio gel bead-in-EMULSIO (gem) sequitur CDNA synthesis

● uterque bead in gemma oneratur cum primers composito ex IV sectiones:

Poly (DT) cauda enim MRNA priming et CDNA synthesis,

Unique mocecular identifier (Umi) ad rectam amplificare bias

10x barcode

Binding Sequence of Partiales Read I Dimer

Commoda

Single-Nucleus RNA sequencing circumvents limitations of una-cell rna sequencing, enabling:

● usum gelida exempla et non solum limited ad recens exempla

● humilis accentus de gelida cellulis cum comparari ad enzymatic curatio nova cellulis, reflectitur in transcriptome notitia in forma minus accentus-adductus genes

● non opus est prior remotionem rubrum sanguinem cellulis

● Unlimited cell diameter

● Magna ordinata ex samples qui sunt eligibile analysis, comprehendo universa et fragilis TEXTUS types qui sunt prone ad cellulam clumping et exitium durante TEXTUS dissociationem

Exempla quae non potest resolvitur per unum-cell rna sequencing et sunt eligibile unum nuclei RNA Sequentia:

Cell / TEXTUS	Ratio
Severe gelida TEXTUS	'Non adepto recens vel diu, salvus in organizations
Musculus cellula, megakaryoyte, adipem ...	Cell diametrum magna intrare instrumentum
Iecoris ...	Nimis fragilis conteram potuit distinguere unum cellulis
Neuron cellula cerebri ...	Magis sensitivo, facile ad accentus, erit mutare in sequencing results
Pancreas, thyroideum ...	Dives in Endogenous enzymes, afficiens productionem unius cellulam suspensionis

Single-nucleus nobis unum-cellulam

SINGULUS	Cellula
Unlimited cell	Diameter cell: 10-40 μm
In materia potest esse gelida TEXTUS	In materia oportet recens TEXTUS
Humilis accentus de gelida cellulis	Enzyme curatio potest causare cell accentus reactionem
Non rubrum sanguinem cellulis postulo ut removeri	Red sanguis cellulis postulo ut removeri
Nuclei exprimit bioinformation	Totum cellula exprimit bioinformation

Cubits

Sample Requirements

Bibliotheca

Sequencing belli

Notitia commendatur

Imperium

Animal TEXTUS ≥ CC mg

Plant TEXTUS ≥ CD mg

10x genomics sn CDNA Library

Illumina pe150

100k PE legit per cellulam

(100-200 GB)

700-1200 nuclei / μl et nuclei integritas servatur in microscopium

Nam More Details on Sample praeparatio ductu et servitium workflow, placet sentire liberum loqui adBMKGene Peritus

Service opus influunt

Previous: Plant / Animal totum Genome sequencing

Next: Genome-wide Association Analysis

Includit sequenti analysis:

● Quality Imperium Number of cellulis, gene deprehensio, accurate idem de cellulis, RNA moleculis et expressio quantitate

● interiorem sample Analysis:

Cellula clustering et botrus annotation

Differentialium Analysis expressio: identificatio in Clusters

Eget Annotation et locupletatione ex botro

● Inter-Group Analysis:

Data compositum

Differentialium expressio Analysis: idem de groups

Eget Annotation et locupletandum Grouping

● Advanced Analysis:

Cellula Cicero analysis

Pseudotime Analysis

Cell Communication Analysis (Cellphonedb)

Gene Set locupletantes Analysis (GSEA)

Interiorem-sample analysis

Cellula clustering:

Differentialium Analysis expressio: botrum portasset

Inter-Group Analysis

Differentialium expressio Analysis: Group Goth

Advanced Analysis:

Pseudotime Analysis:

Cell Cicero Analysis:

Explorarent progressiones facilitati per BMKNGGene scriptor Una-Nucleus RNA Sequencing Services by 10x Chromium in his Featured Publications:

Wang, L. et al. (MMXXI), unum-cell transcriptomic analysis revelat immunes landscape pulmonis in mole-repugnans suspiriosis exacerbation ',Acta de National Academy of Sciences Civitatum Foederatarum Americae, CXVIII (II), p. E2005590118. Doi: 10.1073 / Pnas.2005590118

Zheng, H. et al. (MMXXII) 'a regulatory network pro Dysegulated gene expressio et abnormes metabolicae signalling in immune cellulis in microenvironment de sepulcris' morbus et Hashimoto scriptor thyroiditis ',Fines in immunology, XIII, p. (DCCCLXXIX) DCCCXXIV. Doi: 10,3389 / Fimmu.2022.879824 / Bibtex.

Tian, H. et al. (MMXXIII), una cella transcriptome uncovers heterogeneity et immune respondeo leukocytes post vaccination cum inactivated Edwardsella Tarda in volutpat (Paralichthys Olivaceus),Aquaculture, DLXVI, p. (DCCXXXIX) CCXXXVIII. Doi: 10.1016 / j.Aquaculture.2023.739238.

Yu, Y. et al. (MMXXIII) 'Photodynamic Lorem amplio ad exitus immune LAPIS inhibitors per remodeling anti-tumor immunitatem in aegris cum CARDIACUS cancer',CARDIACUS cancer, XXVI (V), pp. 798-813. Doi: 10.1007 / S10120-023-01409-X / Metrics.