Totum transcriptome sequencing - Illumina
Features
● Dual library to sequence the complete transcriptome: rRNA depletion followed by PE150 library preparation and size selection followed by SE50 bibliotheca praeparatio.
● Analysis bioinformatica completa ex variantibus, lncRNA, circRNA et miRNA in singulis relationibus bioinformaticis
● Iuncturam analysi omnium RNA expressionis in compositione relationis, in reticulis cerNA analysi inclusis.
Servitium Commoda
●Altissimam analysi regulatory retiacula: cerNA analysin retis facultas est per iuncturam sequen- tionis mRNA, lncRNA, circRNA et miRNA et per fluxum operis bioinformaticae volubilitas.
●Comprehensive Annotation: pluribus databases utimur ad officialiter annotate differentialiter expressas Genes (DEGs) et debitam analysin perficiendam, perspicientias processuum cellularum et hypotheticarum quae responsionem transumptam afferunt.
●Expertise: cum indagatio rei bene claudendae super 2000 totum incepta transumptum in variis investigationibus indagandis, turma nostra experientiae opes ad omne propositum affert.
●Rigorous Quality Control: medias potestates efficiendi puncta per omnes aetates, a praepatione specimen et bibliothecam ad sequencias et bioinformaticas.Haec vigilantia exquisita efficit ut traditionem constanter summus qualitas consequitur.
Comprehensive Annotation: pluribus databases utimur ad officialiter annotate differentialiter expressas Genes (DEGs) et debitam analysin perficiendam, perspicientias processuum cellularum et hypotheticarum quae responsionem transumptam afferunt.
●Post-Sales Support: Munus nostrum ultra project complementum cum 3-mensibus post venditionis tempus servitutis extenditur.Hoc tempore consilium praebemus subsequens, fermentum subsidia, et sessiones Q&A compellare quaslibet interrogationes ad eventus relatas.
Sample Requisita et Delivery
| Bibliotheca | Sequencing strategy | Data commendatae | Regimen quālitātis |
| rRNA infrequenti | Illumina PE150 | XVI GB | Q30≥85% |
| Magnitudine electus | Illumina SE50 | 10-20M legit |
Sample Requisita:
Nucleotides:
| Conc. | Moles (μg) | Puritas | Integritas |
| ≥ 100 | ≥ 1 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Nullam dapibus vel dapibus vel DNA contagione gel ostensum est. | Plantae: RIN≥6.5 Animal: RIN≥7.0 5.0≥28S/18S≥1.0; limitata vel non collocantur elevatione |
Sample Delivery commendatur
Continens:
2 ml tubum centrifugium (foil plumbi non commendatur)
Sample labeling: Group+replicata eg A1, A2, A3;B1, B2, B3...
Shipment:
1.Dry-glacies: Exemplaria in saccis referta necesse est et in glacie siccitate defossa.
2.RNAstable tube: RNA specimina in RNA stabilizationis tubo exsiccata possunt (eg RNAstable®) et in cella temperatura exsiccata sunt.
Service Opus O
Experimentum design
Sample traditio
RNA extractionem
Library construction
Sequencing
Analysis
Post-venditionis officia
Bioinformatics
RNA expressio overview
Aliter expressit genes
cerna analysis
Mirnas differentialiter expressit et RNAs affinis
Investigationis progressiones explorare faciliores per BMKGene' operas totum transcriptum sequencium per collectionem publicationum curatam.
Dai, Y. et al.(2022) 'Profationes comprehensivae mRNAs, lncRNAs et miRNAs in Morbus Kashin-Beck, qui a RNA-sequencing notantur', Omics Molecular, 18(2), pp.doi: 10.1039/D1MO00370D.
Liu, N. nan et al.(2022) 'Plena transcriptomes analysis de frigoris resistentiae Apis cerana in Changbai Monte per tempus brumalem.', Gene, 830, pp.doi: 10.1016/J.GENE.2022.146503.
Wang, XJ et al.(2022) 'Multi-omic Integration-Substructio Prioritizationis de Competendi Endogenous RNA Regulation Networks in Small Cell Lung Cancer: Characteres molculares et medicamenti Candidati', Fines in Oncologia, 12, p.904865. doi: 10.3389/FONC.2022.904865/BIBTEX.
Xu, P. et al.(2022) 'Integrae analysi lncRNA/circRNA-miRNA-mRNA expressionis profiles pervestigationes novas in mechanismos potentiales indicat, cum nematodes radicis nodum in peanut', BMC Genomics, 23(1), pp.doi: 10.1186/S12864-022-08470-3/FIGURES/7.
Yan, Z. et al.(2022) 'Omne transcriptome RNA sequens elucidat mechanismos hypotheticos cum sustentatione qualitatis postharvest in algentem red LED irradiationem', Biologia Postharvest et Technology, 188, p.111878. doi: 10.1016/J.POSTHARVBIO.2022.111878.
