Specific Locus Amplified Fragmentum Sequentiae (SLAF-Seq)
Service Details
Technical Scheme
Opus fluxus
Servitium Commoda
Princeps titulum inventionis efficientiam- Princeps throughput sequendi technologiam adiuvat SLAF-Seq in inveniendo centena milia tags in toto genomate.
Humilis dependentia in genome- Applicari potest speciei vel cum genome vel sine comparatione.
Flexibile consilium consilium- Singulus-enzyme, dual-enzyme, multi-enzyme digestio et variae enzymes species, omnes seligi possunt ut diversae investigationis finis seu species opsonare possint.Pre-aestimatio in silico consilio optimalis enzyme asseverare adhibetur.
Enzymatic digestio efficientis- Prae-experimentum factum est ad optimize condiciones, quae experimentum formale firmum ac firmum reddit.Fragmentum collectionis efficientiam super 95% consequi potest.
SLAF tags aequaliter distributa- SLAF tags aequaliter in omnibus chromosomatum maximam partem distribuuntur, attingentes medium 1 SLAF per 4 kb.
Efficax fuga repetit- Sequentia repetita in notitia SLAF-Seq ad inferiora quam 5% reducitur, praesertim in speciebus cum altiori gradu repetitae, ut triticum, spelte, etc.
Extensive experientiaPlus 2000 occlusum SLAF-Seq in centenis inceptis specierum involucris plantarum, mammalium, avium, insectorum, organismi aquatici, etc.
Auto-exculta bioinformatic workflow- Integratio operis bioinformaticae pro SLAF-Seq ab BMKGENE evoluta est, ut certae et accuratae rerum finalium in tuto poneretur.
Service Specifications
| Platform | Conc. | Totalis (ug) | OD260/280 |
| Illumina NovaSeq | >35 | >1.6(Volume>15μl) | 1.6-2.5 |
Commendatur Sequencing Strategy
Altitudo sequentium: 10X/Tag
| Genome Size | Commendatur SLAF Tags |
| <500 Mb | 100K vel WGS' |
| 500 Mb- 1 Gb | 100 K |
| 1 Gb -2 Gb | 200 K |
| Gigas vel complex genomes | 300 - 400K |
| Applications
| Commendatur Ascendite incolarum
| Sequens consilium et profundum
| |
| Profundum
| Numerus Tag
| ||
| GWAS
| Sample numerus ≥ 200
| 10X
|
Secundum genome magnitudine
|
| Evolutionis geneticae
| Individua cuiusque subgroup ≥ 10; summa exemplaria ≥ 30
| 10X
| |
Sample Delivery commendatur
Continens: 2 ml tube centrifugium
Pleraque enim exempla in ethanolo conservare non praecipimus.
Sample labeling: Exemplaria clare intitulata et identificari debent cum forma sample informationis subicienda.
Shipment: siccum-glacies: Exemplaria primum in saccis referta necesse est, et in siccitate glaciei condita.
Service Workflow
Sample QC
Gubernator experimentum
SLAF-experimentum
Bibliotheca Praeparatio
Sequencing
Analysis
Post-venditionis Services
1. Statistics of map result
2. SLAF titulus progressionis
3. Variatio annotationis
| Annus | Journal | IF | Titulus | Applications |
| 2022 | natura communicationum | 17.694 | Fundamentum genomic giga-chromosomata et giga-genome arboris peony Paeonia ostii | SLAF-GWAS |
| 2015 | Novus Phytologus | 7.433 | Domestica vestigia ancoram regionum genomicorum agronomicorum momenti in soybeans | SLAF-GWAS |
| 2022 | Acta Inquisitionis Provectae | 12.822 | Genome-late artificialis introgressiones Gossypium barbadense in G. hirsutum superiores loci manifestare pro emendatione simultanea fibra bombicis qualitas et cede lineamenta | SLAF-Evolutionary genetics |
| 2019 | Planta hypothetica | 10.81 | Populatio Genomic Analysis et De Novo Conventu Originem Weedy Rice ut Evolutionary Ludus | SLAF-Evolutionary genetics |
| 2019 | Natura Genetics | 31.616 | Genome sequence and genetic divers of the common carpus, Cyprinus carpio . | SLAF-Linkage map |
| 2014 | Natura Genetics | 25.455 | Genome eros culti perspectionem leguminum karyotyporum praebet, polyploideum evolutionis et seges domesticae. | SLAF-Linkage map |
| 2022 | Planta Biotechnology Journal | 9.803 | Lepidium sativum ST1 indicat de seminis morphologiam hitchhiking delectu involvente et oleum content in soybean domestica | SLAF-Marker progressio |
| 2022 | Acta Internationalis Scientiarum Molecularis | 6.208 | Lepidium sativum et DNA Development pro tritico-Leymus 2Ns (2D) Disomic chromosomatum Substitutio | SLAF-Marker progressio |
