Metagenomic Sequencing, TGS
A Metagenome est collectio ex geneticae materia de mixta civitatem organismis, ut environmental et humana Metagenomiis. Contineat genomes et cultu et uncultivatizati microorganisms. Metagenomic sequencing enables studium harum intricatus genomic landscapes embedded in adipiscing samples a providente plus quam taxonomic profiling. Hoc quoque oblatione genomicos perspective per exploring in encoded genes et putative roles in environmental processus. Dum traditional shotgun aditus cum Illumina sequencing sunt late in Metagenomic studiis, adventum Nanopore et PacBio Long-lego sequencing mutata agro. Nanopore et PacBio technology augendae downstream bioinformatic analyses, notabiliter Metagenome Conventus, Concordibus continuam. Renuntiationes indicant quod Nanopore, secundum quod PacBio-secundum Metagenomics feliciter generatae completum et clausit bacterial genomes ex complexu microbiomes (Moss, El, et al., Natura Biotech, MMXX). Integrating Nanopore legit cum Illumina legit providet opportuna accedens errorem disciplinam, mitigating nanopore scriptor inhaerens humilis accurate. Hoc synergistic combination leverages vires cuiusque sequencing platform, offering robust solutio superare potential limitations et progredientes praecisione et reliability of Metagenomic analyzes.
Platform: Nanopore Promethion XLVIII, Illumia et PacBio Revio
Service Features
● diu legit sequencing in Nanopore P48 (10kb Library); PacBio Revio (8kb Library)
● Error-correction: sequencing in illumina novaseq (Pe150 Library)
Service commoda
●High Quality Metagenome Conventus:Cum paucioribus contigs quod habent altius continuationem.
●Superiore accurate:Species idem et eget gene praedictum.
●Improved conventus: Facilitating bacterial Genome Setration et comparative Metagenome Analysis.
●Comprehensive Bioinformatics Analysis:Nostrum analysis est focused non solum in taxonomic diversitas sed etiam in civitatem in operatione diversitas.
●Extensive experientia: Nostrum quadrigis affert opes experientia ad singulis project, cum track record of prospere claudendo multiple Metagenomics projects in variis investigationis domains et processui super 200,000 exempla, nostra quadrigis affert divitiis per experientiam omnibus project.
Service Specifications
| Sequencing platform | Sequencing belli | Notitia commendatur |
| Illumina novaseq | Pe150 | VI GB |
| Nanopore P48 | X KB | VI / X GB |
| PacBio Revio | VIII KB | X / XX GB |
Sample Requirements
| Concentration (ng / μl) | Summa copia (μg) | Volubilis (μl) | |
| Illumina Pe150 Library | ≥1 | ≥0.03 | ≥20 |
| Nanopore X MB Library | ≥40 | ≥2 | ≥20 |
| PacBio VIII MB Library | ≥50 | X μg / Cell | ≥20 |
Service opus influunt
Sample partum
Constructio
Sequencing
Analysis
Post-Sale Services
Includit sequenti analysis:
● Data qualis potestate sequencing
● Metagenome Conventus et Gene Prediction
● gene annotation
● Taxonomic Alpha diversitas analysis
● eget analysis de Community: Biological munus, metabolicae, antibiotic resistentia
● analysis in utroque eget et taxonomic diversitas:
Beta diversitas analysis
Inter-Group Analysis
Correlation Analysis inter environmental factores et compositionem et diversitatem
Non-redundant gene set distribution:
Gene Annotation: KEGGIA PRO
Gene Annotation: eggnog
Gene Annotation: Campy Carbohydrates
Species Correlation Network:
Explorarent progressiones facilitati per BMKGene scriptor Metagenomics sequencing Service cum Nanopore + illumina hoc Featured publication:
Jin, H. et al. (MMXXIII) 'High-Genome Compendium Humani Gut microbiome de interiore Mongolians', natura microbiology MMXXIII VIII: I, VIII (I), pp. 150-161. Doi: 10.1038 / S41564-02201270-1.
