Dum non-coding sequencet, illumina
Service commoda
●Iuncturam analysis de mRNA et lncrnaPer combining quantitatem mRNA transcripts cum studiis Lncrna et eorum peltas, est possibile ut an in-profundum Overview de regulatory mechanism underlying cellular responsum.
●Extensive expertise: Nostrum quadrigis affert opes experientia ad singulis project, cum track record of processus super 23.000 exempla in BMK spannning diverse sample genera et lncrna projects.
●Rigorous species imperiumNos effectum deducendi core imperium punctorum per omnes gradus, ex sample et bibliotheca praeparatio ad sequencing et bioinformatics. Hoc methutus vigilantia ensures ad partum constanter summus qualitas praecessi.
●Post-Sales Support: Our commitment extendat ultra project complementum cum III-mensis post-Sale Service tempus. Per hoc tempus, ut offerimus project sequantur, sursum, Troubleshooting auxilium, et Q & sessiones ad oratio quis queries ad praecessi.
Sample requisitis et partus
| Bibliotheca | Suggestus | Commendatur notitia | Data QC |
| RRNA infrequenti directional Library | Illumina pe150 | 10-16 GB | Q30≥85% |
Nucleotides:
| Conc. (NG / μl) | Moles (μg) | Pudicitia | Integritas |
| ≥ LXXX | ≥ 0.8 | OD260 / CCLXXX = 1.7-2.5 OD260 / CCXXX = 0.5-2.5 Limited vel dapibus vel DNA contamination ostensum est in Gel. | Rin≥6.0; 5.0≥28s / 18s≥1.0; limited aut non baseline elevatio |
● plantis:
Radix, caule aut Fem petal: CDL mg
Folium semen, CCC mg
Fructus: 1.2 G
● animalis:
Cordis et intestinum: CDL mg
Viscera vel cerebrum: CCXL mg
Musculus: DC mg
Ossa, capillos et cutis: 1.5g
● Arthropods:
Insecta: 9G
Crustacea: CDL mg
● totum sanguinem:II tubulis
● cellulis: 106 cellulae
● Serum et PLASMA; VI ml
Commendatur Sample partus
Continens: II ml Centrifuge tubo (stagnum ffoyle non commendatur)
Sample Labeling: Group + replicate EG A1 A2, A3; B1, B2, B3.
Shipment:
I. Arida, Ice: Exempla postulo ut facis in sacculos et sepulta in sicco-glacies.
II. Rnastable tubulis: RNA exempla potest siccari in RNA stabilization tubo (eg rnastable®) et uit in locus temperatus.
Service opus influunt
Experimentum Design
Sample partum
RNA extraction
Constructio
Sequencing
Analysis
Post-Sale Services
Bioinformatics
Diversus gene expressio (gradus) analysis
Quantum de Lncrna expressio - clustering
Loca locat Lncrna target
MRNA et LnCrna Position Analysis - Circos insidiamini (Medius MRNA et interiore cicrlce est lncrna)
Explorarent progressiones facilitati BMKGene scriptor Lncrna aequinque Services per curated collectione publications.
Ji, H. et al. (MMXX), identificatio, eget praedictio, et key lncrna verificationem de frigore accentus-related lncrnas in mures iecoris ', scientific tradit MMXX X: I, X (I), pp. 1-14. Doi: 10.1038 / S41598-020-57451-7.
Jia, Z. et al. (MMXXI) 'integrative transcriptomic analysis revelat immune mechanism ad Cyhv-III-repugnans communi carp contentionem', fines in immunology, XII, p. (DCLXXXVII) CLI. Doi: 10,3389 / Fimmu.201.687151 / BibTex.
Wang, XJ et al. (MMXXII), Multi-omics integration-fundatur prioritization de competing endogenous RNA moderari networks in parva cellula pulmone cancer: Molecular et medicamento candidati ', in oncology, XII, p. (CMIV) DCCCLXV. Doi: 10,3389 / Fonc.2022.904865 / Bibtex.
Xiao, L. et al. (MMXX) 'geneticae dissectione gene coxpression network underlying photosynthesis in Populus, Plant Biotechnology Journal, XVIII (IV), pp. 1015-1026. Doi: 10.11111 / Pbi.13270.
Zheng, H. et al. (MMXXII) 'a regulatory network pro Descysregulated Gene expressio et abnormes metabolicae signum in immune cellulis in microenvironment de sepulcris' morbus et Hashimoto scriptor thyroiditis ', in immunology, XIII, p. (DCCCLXXIX) DCCCXXIV. Doi: 10,3389 / Fimmu.2022.879824 / Bibtex.
