T2T INTEKO ISHINGA AMATEGEKO, GENOME YUBUNTU
1stGenome ebyiri z'umuceri1
Umutwe: Guteranya no Kwemeza Ibice bibiri bidafite aho bihuriye na Xian / indica Umuceri Ugaragaza Ubushishozi Mububiko bwa Centromere
Doi:https://doi.org/10.1101/2020.12.24.424073
Igihe cyashyizweho: 01 Mutarama 2021.
Ikigo: Kaminuza y’ubuhinzi ya Huazhong, mu Bushinwa
Ibikoresho
O. sativa xian / indicaubwoko bwumuceri 'Zhenshan 97 (ZS97)' na 'Minghui 63 (MH63)
Ingamba zikurikirana
NGS isoma + HiFi isoma + CLR isoma + BioNano + Hi-C
Amakuru:
ZS97: 8.34 Gb （~ 23x） HiFi isoma + 48.39 Gb (~ 131x) CLR isoma + 25 Gb (~ 69x) NGS + 2 BioNano Irys selile
MH63: 37.88 Gb (~ 103x) HiFi isoma + 48.97 Gb (~ 132x) CLR isoma + 28 Gb (~ 76x) NGS + 2 BioNano Irys selile
Igishushanyo 1 Genome ebyiri zidafite icyuho cyumuceri (MH63 na ZS97)
2ndBanana Genome2
Umutwe: Telomere-kuri-telomere ikinyuranyo cya chromosomes yigitoki ukoresheje nanopore ikurikirana
Doi:https://doi.org/10.1101/2021.04.16.440017
Igihe cyashyizweho: 17 Mata 2021.
Ikigo: Université Paris-Saclay, Ubufaransa
Ibikoresho
Kabiri haploidMusa acuminatasppmalaccensis(DH-Pahang)
Ingamba zikurikirana hamwe namakuru:
MurahoSeq2500 PE250 uburyo + MinION / Isezerano (93Gb, ~ 200X) + Ikarita nziza (DLE-1 + BspQ1)
Imbonerahamwe 1 Kugereranya inteko ya genome ya Musa (DH-Pahang)
Igishushanyo cya 2 Musa genomes igereranya
3rdPhaeodactylum tricornutum genome3
Umutwe: Telomere-kuri-telomere inteko ya genome yaP
haeodactylum tricornutum
Doi:https://doi.org/10.1101/2021.05.04.442596
Igihe cyashyizweho: Ku ya 04 Gicurasi 2021
Ikigo: Kaminuza y'Iburengerazuba, Kanada
Ibikoresho
Phaeodactylum tricornutum(Icyegeranyo cy'umuco cya Algae na Protozoa CCAP 1055/1)
Ingamba zikurikirana hamwe namakuru:
1 Oxford Nanopore minion itemba + a 2 × 75 ihujwe-iherezo hagati-isohoka NextSeq 550 ikora
Igishushanyo cya 3 Igikorwa cya telomere-kuri-telomere inteko ya genome
4thUmuntu CHM13 genome4
Umutwe: Urutonde rwuzuye rwa genomuntu
Doi:https://doi.org/10.1101/2021.05.26.445798
Igihe cyashyizweho: 27 Gicurasi 2021
Ikigo: Ikigo cyigihugu cyubuzima (NIH), Amerika
Ibikoresho: umurongo w'akagari CHM13
Ingamba zikurikirana hamwe namakuru:
30 × Urutonde rwa PacBio ruzenguruka (HiFi), 120 × Oxford Nanopore ultra-ndende ikurikirana, 100 × Illumina PCR-Yubusa (ILMN), 70 × Illumina / Arima Genomics Hi-C (Hi-C), Ikarita ya optique ya BioNano, na Ikurikiranyabihe
Imbonerahamwe 2 Kugereranya GRCh38 na T2T-CHM13 inteko rusange yabantu
Reba
1.Sergey Nurk n'abandi.Urukurikirane rwuzuye rwa genomuntu.bioRxiv 2021.05.26.445798;doi:https://doi.org/10.1101/2021.05.26.445798
2.Caroline Belser n'abandi.Telomere-kuri-telomere icyuho cya chromosomes yigitoki ukoresheje nanopore ikurikirana.bioRxiv 2021.04.16.440017;doi:https://doi.org/10.1101/2021.04.16.440017
3.Daniel J. Giguere n'abandi.Telomere-kuri-telomere inteko ya Phaeodactylum tricornutum.bioRxiv 2021.05.04.442596;doi:https://doi.org/10.1101/2021.05.04.442596
4. Indirimbo ya Jia-Ming n'abandi.Inteko no Kwemeza Ibice bibiri bidafite aho bihuriye na Xian / indica Umuceri Ugaragaza Ubushishozi Mububiko bwa Centromere.bioRxiv 2020.12.24.424073;doi:https://doi.org/10.1101/2020.12.24.424073
Igihe cyo kohereza: Mutarama-06-2022