T2T GENOME Majalisar, GAP FREE GENOME
1stBiyu Shinkafa Genome1
Take: Tattaunawa da Tabbatar da Nassosi Biyu marasa Rata don Xian/indica Shinkafa Ya Bayyana Hazaka kan Gine-ginen Tsirrai Centromere
Doi:doi.org/10.1101/2020.12.24.424073
Lokacin Buga: Janairu 01, 2021.
Cibiyar: Jami'ar aikin gona ta Huazhong, kasar Sin
Kayayyaki
O. sativa xian/indicairin shinkafa 'Zhenshan 97 (ZS97)' da 'Minghui 63 (MH63)
Dabarun jeri
NGS karanta + HiFi karanta + CLR karanta + BioNano + Hi-C
Bayanai:
ZS97: 8.34 Gb (~ 23x) HiFi yana karanta + 48.39 Gb (~ 131x) CLR yana karanta + 25 Gb (~ 69x) NGS + 2 BioNano Irys Kwayoyin
MH63: 37.88 Gb (~ 103x) HiFi yana karanta + 48.97 Gb (~ 132x) CLR yana karanta + 28 Gb(~76x) NGS + 2 BioNano Irys sel
Hoto 1 Genomes guda biyu na shinkafa mara rata (MH63 da ZS97)
2ndBanana Genome2
Take: Telomere-to-telomere chromosomes na ayaba marasa gata ta amfani da jerin nanopore
Doi:doi.org/10.1101/2021.04.16.440017
An buga lokaci: Afrilu 17, 2021.
Cibiyar: Jami'ar Paris-Saclay, Faransa
Kayayyaki
Haploid biyuMusa acuminatasppmalaccensis(DH-Pahang)
Tsarin tsari da bayanai:
Yanayin HiSeq2500 PE250 + MinION/ PromethION (93Gb, ~ 200X)+ Taswirar gani (DLE-1+BspQ1)
Table 1 Kwatanta Musa acuminata (DH-Pahang) genome majalisai
Hoto na 2 Musa genomes kwatancen gine-gine
3rdPhaeodactylum tricornutum genome3
Take: Telomere-to-telomere genome meeting ofP
haeodactylum tricornutum
Doi:doi.org/10.1101/2021.05.04.442596
An buga lokaci: Mayu 04, 2021
Cibiyar: Western University, Kanada
Kayayyaki
Pheodactylum tricornutum(Tarin Algae da Protozoa CCAP 1055/1)
Tsarin tsari da bayanai:
1 Oxford Nanopore minION tantanin halitta + 2 × 75 mai haɗa-karshen ƙarshen fitarwa NextSeq 550 gudu
Hoto na 3 Gudun Aiki don taron genome-to-telomere
4thKwayoyin halittar CHM134
Take: Cikakken jerin kwayoyin halittar dan adam
Doi:doi.org/10.1101/2021.05.26.445798
An buga lokaci: Mayu 27, 2021
Cibiyar: Cibiyar Kiwon Lafiya ta Kasa (NIH), Amurka
Kayan aiki: layin salula CHM13
Tsarin tsari da bayanai:
30 × PacBio madauwari yarjejeniya (HiFi), 120 × Oxford Nanopore ultra-long read sequencing, 100 × Illumina PCR-Free sequencing (ILMN), 70 × Illumina / Arima Genomics Hi-C (Hi-C), BioNano Tantancewar taswira, da Strand-seq
Tebura 2 Kwatanta GRCh38 da T2T-CHM13 taron kwayoyin halittar ɗan adam
Magana
1. Sergey Nurk et al.Cikakken jerin kwayoyin halittar ɗan adam.bioRxiv 2021.05.26.445798;doi:doi.org/10.1101/2021.05.26.445798
2. Caroline Belser et al.Telomere-zuwa-telomere chromosomes na ayaba marasa gata ta amfani da jerin nanopore.bioRxiv 2021.04.16.440017;doi:doi.org/10.1101/2021.04.16.440017
3. Daniel J. Giguere et al.Telomere-to-telomere genome taro na Phaeodactylum tricornutum.bioRxiv 2021.05.04.442596;doi:doi.org/10.1101/2021.05.04.442596
4. Jia-Ming Song et al.Tattaunawa da Tabbatar da Bayanan Halitta guda biyu marasa rata don Xian/indica Shinkafa ya Bayyana Hakika cikin Tsarin Gine-gine na Centromere.bioRxiv 2020.12.24.424073;doi:doi.org/10.1101/2020.12.24.424073
Lokacin aikawa: Janairu-06-2022