T2T GENOME ASSEMBLY, GAP FREE GENOME
1stTwa Rice Genomes1
Titel: Gearstalling en falidaasje fan twa gap-frije referinsjegenoom foar Xian / indica Rice ûntbleatet ynsjoch yn plant Centromere Architecture
Doi:https://doi.org/10.1101/2020.12.24.424073
Pleatst tiid: 01 jannewaris 2021.
Ynstitút: Huazhong Agricultural University, Sina
Materialen
O. sativa xian/indicarysrassen 'Zhenshan 97 (ZS97)' en 'Minghui 63 (MH63)
Sequencing strategy
NGS lêst + HiFi-lêzen + CLR-lêzen + BioNano + Hi-C
Data:
ZS97: 8.34 Gb(~23x) HiFi-lêzen + 48.39 Gb (~131x) CLR-lêzen + 25 Gb(~69x) NGS + 2 BioNano Irys-sellen
MH63: 37,88 Gb (~103x) HiFi-lêzen + 48,97 Gb (~132x) CLR-lêzen + 28 Gb (~76x) NGS + 2 BioNano Irys-sellen
Ofbylding 1 Twa gap-frije genomen fan rys (MH63 en ZS97)
2ndBanana Genome2
Titel: Telomere-to-telomere gapless chromosomes of banana using nanopore sequencing
Doi:https://doi.org/10.1101/2021.04.16.440017
Tiid pleatst: 17 april 2021.
Ynstitút: Université Paris-Saclay, Frankryk
Materialen
Dûbel haploidMusa acuminatasppmalacensis(DH-Pahang)
Sequence strategy en gegevens:
HiSeq2500 PE250 modus + MinION/ PromethION (93Gb,~200X)+ Optyske kaart (DLE-1+BspQ1)
Tabel 1 Ferliking fan Musa acuminata (DH-Pahang) genome gearkomsten
figuer 2 Musa genomes arsjitektuer ferliking
3rdPhaeodactylum tricornutum genome3
Titel: Telomere-to-telomere genome gearstalling fanP
haeodactylum tricornutum
Doi:https://doi.org/10.1101/2021.05.04.442596
Pleatst tiid: 04 maaie 2021
Ynstitút: Western University, Kanada
Materialen
Phaeodactylum tricornutum(Culture Collection of Algae and Protozoa CCAP 1055/1)
Sequence strategy en gegevens:
1 Oxford Nanopore minION flow sel + in 2 × 75 paired-end mid-output NextSeq 550 run
Ofbylding 3 Workflow foar telomere-to-telomere genome-assemblage
4thHuman CHM13 genoom4
Titel: De folsleine folchoarder fan in minsklik genoom
Doi:https://doi.org/10.1101/2021.05.26.445798
Pleatst tiid: 27 maaie 2021
Ynstitút: National Institutes of Health (NIH), Feriene Steaten
Materiaal: selline CHM13
Sequence strategy en gegevens:
30 × PacBio sirkulêre konsensus-sequencing (HiFi), 120 × Oxford Nanopore ultra-lange lêssekwinsje, 100 × Illumina PCR-frije sequencing (ILMN), 70 × Illumina / Arima Genomics Hi-C (Hi-C), BioNano Genomics Hi-C (Hi-C) en Strand-seq
Tabel 2 Fergeliking fan GRCh38 en T2T-CHM13 minsklike genome assemblies
Referinsje
1. Sergey Nurk et al.De folsleine folchoarder fan in minsklik genoom.bioRxiv 2021.05.26.445798;doi:https://doi.org/10.1101/2021.05.26.445798
2. Caroline Belser et al.Telomere-to-telomere gapless chromosomen fan banaan mei help fan nanopore sequencing.bioRxiv 2021.04.16.440017;doi:https://doi.org/10.1101/2021.04.16.440017
3. Daniel J. Giguere et al.Telomere-to-telomere genome assembly of Phaeodactylum tricornutum.bioRxiv 2021.05.04.442596;doi:https://doi.org/10.1101/2021.05.04.442596
4. Jia-Ming Song et al.Gearstalling en falidaasje fan twa Gap-frije referinsje genomes foar Xian / indica Rice ûntbleatet ynsjoch yn Plant Centromere Architecture.bioRxiv 2020.12.24.424073;doi:https://doi.org/10.1101/2020.12.24.424073
Post tiid: Jan-06-2022