T2T GENOME ASSEMBLY, GAP FREE GENOME
1stRua Raihi Genomes1
Taitara: Huihuinga me te Whakamanatanga o nga Ira Tohutoro Ake-kore e rua mo te Raihi Xian/indica e whakaatu ana i nga tirohanga ki te Hangahanga Tipu Centromere
Doi:https://doi.org/10.1101/2020.12.24.424073
Wā Whakairi: Hanuere 01, 2021.
Te Whare Wananga: Huazhong Agricultural University, Haina
Rauemi
O. sativa xian/indicamomo raihi 'Zhenshan 97 (ZS97)' me 'Minghui 63 (MH63)
Rautaki raupapa
Ka panui a NGS + Ka panui HiFi + Ka panui CLR + BioNano + Hi-C
Raraunga:
ZS97: 8.34 Gb（~23x）HiFi panui + 48.39 Gb (~131x ) CLR panui + 25 Gb(~69x) NGS + 2 BioNano Irys pūtau
MH63: 37.88 Gb (~103x) HiFi panui + 48.97 Gb (~132x) CLR panui + 28 Gb(~76x) NGS + 2 BioNano Irys pūtau
Whakaahua 1 E rua nga momo ira raihi kore-aputa (MH63 me ZS97)
2ndPanana Genome2
Title: Telomere-to-telomere gapless chromosomes of banana using nanopore sequencing
Doi:https://doi.org/10.1101/2021.04.16.440017
Wā Whakairi: Paenga-whāwhā 17, 2021.
Te Whare Wananga: Université Paris-Saclay, France
Rauemi
haploid ruaMusa acuminatasppmalaccensis(DH-Pahang)
Rautaki raupapa me nga raraunga:
Aratau HiSeq2500 PE250 + MinION/ PromethION (93Gb,~200X )+ Mahere Optical (DLE-1+BspQ1)
Ripanga 1 Whakatauritenga o nga huihuinga ira tangata a Musa acuminata (DH-Pahang).
Whakaahua 2 Musa genomes hoahoanga whakataurite
3rdPhaeodactylum tricornutum genome3
Title: Telomere-to-telomere genome assembly ofP
haeodactylum tricornutum
Doi:https://doi.org/10.1101/2021.05.04.442596
Wā Whakairi: Mei 04, 2021
Te Whare Wananga: Western University, Canada
Rauemi
Phaeodactylum tricornutum(Kohinga Ahurea o te Pukohu me te Protozoa CCAP 1055/1)
Rautaki raupapa me nga raraunga:
1 Oxford Nanopore miniION pūtau rere + he 2×75 takirua-whakamutunga waenganui-putanga NextSeq 550 oma
Whakaatu 3 Reremahi mo te whakahiato ira telomere-ki-telomere
4thTangata CHM13 genome4
Title: Te raupapa katoa o te ira tangata
Doi:https://doi.org/10.1101/2021.05.26.445798
Wā Whakairi: Mei 27, 2021
Institute: National Institutes of Health (NIH), USA
Rauemi: raina pūtau CHM13
Rautaki raupapa me nga raraunga:
30× PacBio circular consensus sequencing (HiFi) , 120× Oxford Nanopore ultra-long read sequencing , 100× Illumina PCR-Free sequencing (ILMN) , 70× Illumina / Arima Genomics Hi-C (Hi-C), BioNano mapi whatu, me te Strand-seq
Ripanga 2 Whakatauritenga o nga huihuinga ira tangata GRCh38 me T2T-CHM13
Tohutoro
1.Sergey Nurk et al.Ko te raupapa katoa o te ira tangata.bioRxiv 2021.05.26.445798;doi:https://doi.org/10.1101/2021.05.26.445798
2.Caroline Belser et al.Telomere-ki-telomere kawakore o nga chromosomes o te panana e whakamahi ana i te raupapa nanopore.bioRxiv 2021.04.16.440017;doi:https://doi.org/10.1101/2021.04.16.440017
3.Daniel J. Giguere et al.Telomere-to-telomere genome assembly of Phaeodactylum tricornutum.bioRxiv 2021.05.04.442596;doi:https://doi.org/10.1101/2021.05.04.442596
4.Jia-Ming Waiata et al.Ko te Huihuinga me te Whakamana i nga Ira Tohutoro Ake-kore e rua mo te Raihi Xian/indica e whakaatu ana i nga tirohanga ki te Hangahanga Centromere Plant.bioRxiv 2020.12.24.424073;doi:https://doi.org/10.1101/2020.12.24.424073
Te wa tuku: Hanuere-06-2022