T2T GENOME ASSEMBLY, GAP FREE GENOME
1stDalawang Rice Genome1
Pamagat: Pagpupulong at Pagpapatunay ng Dalawang Gap-free Reference Genome para sa Xian/indica Rice ay Nagpapakita ng Mga Insight sa Plant Centromere Architecture
Doi:https://doi.org/10.1101/2020.12.24.424073
Oras ng Pag-post: Enero 01, 2021.
Institute: Huazhong Agricultural University, China
Mga materyales
O. sativa xian/indicauri ng palay 'Zhenshan 97 (ZS97)' at 'Minghui 63 (MH63)
Diskarte sa pagkakasunud-sunod
Ang NGS reads + HiFi reads + CLR reads + BioNano + Hi-C
Data:
ZS97: 8.34 Gb（~23x）HiFi reads + 48.39 Gb (~131x ) CLR reads + 25 Gb(~69x) NGS + 2 BioNano Irys cells
MH63: 37.88 Gb (~103x) HiFi reads + 48.97 Gb (~132x) CLR reads + 28 Gb(~76x) NGS + 2 BioNano Irys cells
Larawan 1 Dalawang walang puwang na genome ng bigas (MH63 at ZS97)
2ndGenome ng Saging2
Pamagat: Telomere-to-telomere gapless chromosome ng saging gamit ang nanopore sequencing
Doi:https://doi.org/10.1101/2021.04.16.440017
Oras ng Na-post: Abril 17, 2021.
Institute: Université Paris-Saclay, France
Mga materyales
Dobleng haploidMusa acuminatasppmalaccensis(DH-Pahang)
Diskarte at data ng sequencing:
HiSeq2500 PE250 mode + MinION/ PromethION (93Gb,~200X )+ Optical na mapa (DLE-1+BspQ1)
Talahanayan 1 Paghahambing ng Musa acuminata (DH-Pahang) genome assemblies
Figure 2 Paghahambing ng arkitektura ng Musa genomes
3rdPhaeodactylum tricornutum genome3
Pamagat: Telomere-to-telomere genome assembly ngP
haeodactylum tricornutum
Doi:https://doi.org/10.1101/2021.05.04.442596
Oras ng Pag-post: Mayo 04, 2021
Institute: Western University, Canada
Mga materyales
Phaeodactylum tricornutum(Kultura na Koleksyon ng Algae at Protozoa CCAP 1055/1)
Diskarte at data ng sequencing:
1 Oxford Nanopore minION flow cell + isang 2×75 paired-end mid-output na NextSeq 550 run
Figure 3 Workflow para sa telomere-to-telomere genome assembly
4thHuman CHM13 genome4
Pamagat: Ang kumpletong pagkakasunud-sunod ng isang genome ng tao
Doi:https://doi.org/10.1101/2021.05.26.445798
Oras ng Pag-post: Mayo 27, 2021
Institute: National Institutes of Health (NIH), USA
Mga Kagamitan: cell line CHM13
Diskarte at data ng sequencing:
30× PacBio circular consensus sequencing (HiFi) , 120× Oxford Nanopore ultra-long read sequencing , 100× Illumina PCR-Free sequencing (ILMN) , 70× Illumina / Arima Genomics Hi-C (Hi-C), BioNano optical maps, at Strand-seq
Talahanayan 2 Paghahambing ng GRCh38 at T2T-CHM13 human genome assemblies
Sanggunian
1.Sergey Nurk et al.Ang kumpletong pagkakasunud-sunod ng isang genome ng tao.bioRxiv 2021.05.26.445798;doi:https://doi.org/10.1101/2021.05.26.445798
2.Caroline Belser et al.Telomere-to-telomere gapless chromosome ng saging gamit ang nanopore sequencing.bioRxiv 2021.04.16.440017;doi:https://doi.org/10.1101/2021.04.16.440017
3.Daniel J. Giguere et al.Telomere-to-telomere genome assembly ng Phaeodactylum tricornutum.bioRxiv 2021.05.04.442596;doi:https://doi.org/10.1101/2021.05.04.442596
4.Jia-Ming Song et al.Ang Assembly at Validation ng Dalawang Gap-free Reference Genome para sa Xian/indica Rice ay Nagpapakita ng Mga Insight sa Plant Centromere Architecture.bioRxiv 2020.12.24.424073;doi:https://doi.org/10.1101/2020.12.24.424073
Oras ng post: Ene-06-2022