T2T GENOME ASSEMBLY, GAP FREE GENOME
1stLaba nooc oo bariis ah1
Ciwaanka: Isku imaatinka iyo Ansixinta Labada Genom ee Tixraaca bilaa Farqiga ah ee Xian/indica Bariiska ayaa daaha ka qaaday aragtida Dhismaha Centromere ee dhirta
Doi:https://doi.org/10.1101/2020.12.24.424073
Waqtiga la dhejiyay: Janaayo 01, 2021.
Machadka: Huazhong Agricultural University, Shiinaha
Qalabka
O. sativa xian/indicaNoocyada bariiska 'Zhenshan 97 (ZS97)' iyo 'Minghui 63 (MH63)
Istaraatiijiyada isku xigxiga
NGS akhriya + HiFi akhriya + CLR akhriya + BioNano + Hi-C
Xogta:
ZS97: 8.34 Gb (~ 23x) HiFi wuxuu akhriyaa + 48.39 Gb (~ 131x) CLR akhriya + 25 Gb (~ 69x) NGS + 2 Unugyada BioNano Irys
MH63: 37.88 Gb (~ 103x) HiFi akhriya + 48.97 Gb (~132x) CLR akhriya + 28 Gb(~76x) NGS + 2 BioNano Irys unugyo
Jaantuska 1 Laba genome oo bariis ah oo aan faraq lahayn (MH63 iyo ZS97)
2ndMuuska Genome2
Ciwaanka: Telomere-ilaa-telomere koromosoomyada muuska ee aan gaabnayn iyadoo la isticmaalayo isku xigxiga nanopore
Doi:https://doi.org/10.1101/2021.04.16.440017
Waqtiga la dhejiyay: Abriil 17, 2021.
Machadka: Jaamacadda Paris-Saclay, Faransiiska
Qalabka
Haploid laba jibaaranMuuse acuminatasppmalaccensis(DH-Pahang)
Istaraatiijiyada isku xigxiga iyo xogta:
Qaabka HiSeq2500 PE250 + MinION/ PromethION (93Gb,~200X)+ Khariidadda indhaha (DLE-1+BspQ1)
Shaxda 1 Isbarbardhigga Musa acuminata (DH-Pahang) genome assemblies
Jaantuska 2 Musa genomes isbarbardhigga naqshadaha
3rdPheodactylum tricornutum genome3
Ciwaanka: Shirarka genome-ka Telomere-to-telomere eeP
haeodactylum tricornutum
Doi:https://doi.org/10.1101/2021.05.04.442596
Waqtiga la dhejiyay: Meey 04, 2021
Machadka: Western University, Canada
Qalabka
Pheodactylum tricornutum(Ururinta Dhaqanka Algae iyo Protozoa CCAP 1055/1)
Istaraatiijiyada isku xigxiga iyo xogta:
1 Oxford Nanopore minION unugga qulqulka + 2 × 75-dhamaadka-dhamaadka wax soo saarka NextSeq 550
Jaantuska 3 Socodka shaqada ee isu-ururinta genome-to-telomere
4thHuman CHM13 genome4
Ciwaanka: Isku xigxiga oo dhammaystiran ee hiddo-wadaha aadanaha
Doi:https://doi.org/10.1101/2021.05.26.445798
Waqtiga la dhejiyay: Meey 27, 2021
Machadka: Machadka Qaranka ee Caafimaadka (NIH), USA
Qalabka: xariiqda gacanta CHM13
Istaraatiijiyada isku xigxiga iyo xogta:
30 × PacBio wareegtada isku xigxiga isku raacitaanka (HiFi), 120 × Oxford Nanopore taxanaha akhriska aadka u dheer, 100 × Illumina PCR-taxane bilaash ah (ILMN) , 70 × Illumina / Arima Genomics Hi-C (Hi-C), Khariidadaha indhaha ee BioNano, iyo Strand-seq
Shaxda 2 Isbarbardhigga GRCh38 iyo T2T-CHM13
Tixraac
1.Sergey Nurk iyo al.Tixda dhamaystiran ee genome-ka aadanaha.bioRxiv 2021.05.26.445798;doi:https://doi.org/10.1101/2021.05.26.445798
2.Caroline Belser iyo al.Telomere-ilaa-telomere koromosoomyada muuska ee aan gaabska lahayn iyadoo la adeegsanayo isku xigxiga nanopore.bioRxiv 2021.04.16.440017;doi:https://doi.org/10.1101/2021.04.16.440017
3.Daniel J. Giguere iyo al.Kulanka genome-ka Telomere-to-telomere ee Pheodactylum tricornutum.bioRxiv 2021.05.04.442596;doi:https://doi.org/10.1101/2021.05.04.442596
4.Jia-Ming Song iyo al.Isu imaatinka iyo Ansixinta Labada Genom ee Tixraaca bilaa Farqiga ah ee Xian/indica Rice ayaa daaha ka qaaday aragtida Dhismayaasha Centromere ee Dhirta.bioRxiv 2020.12.24.424073;doi:https://doi.org/10.1101/2020.12.24.424073
Waqtiga boostada: Jan-06-2022