T2T GENOME ASSEMBLY, GAP FREE GENOME
1stLua Alaisa Genomes1
Igoa: Fa'apotopotoga ma Fa'amaoniga o Genomes Fa'asinoga e Lua Gap-free mo Xian/indica Rice Fa'aalia Malamalamaga i La'au Centromere Architecture
Doi:https://doi.org/10.1101/2020.12.24.424073
Taimi Fa'asalalau: Ianuari 01, 2021.
Inisetiute: Huazhong Agricultural University, Saina
Meafaitino
O. sativa xian/indicaituaiga araisa 'Zhenshan 97 (ZS97)' ma 'Minghui 63 (MH63)
Fuafuaga fa'asologa
Faitau NGS + faitau HiFi + faitau CLR + BioNano + Hi-C
Fa'amatalaga:
ZS97: 8.34 Gb（~23x）HiFi faitau + 48.39 Gb (~131x) CLR faitau + 25 Gb(~69x) NGS + 2 BioNano Irys sela
MH63: 37.88 Gb (~103x) HiFi faitau + 48.97 Gb (~132x) CLR faitau + 28 Gb(~76x) NGS + 2 BioNano Irys sela
Ata 1 E lua genome e leai se avanoa o araisa (MH63 ma ZS97)
2ndFa'i Genome2
Igoa: Telomere-i-telomere gapless chromosomes o fa'i fa'aaoga le nanopore sequencing
Doi:https://doi.org/10.1101/2021.04.16.440017
Taimi Fa'asalalau: Aperila 17, 2021.
Inisetiute: Université Paris-Saclay, Farani
Meafaitino
Fa'alua haploidMusa acuminatasppmalaccensis(DH-Pahang)
Fa'asologa o fuafuaga ma fa'amaumauga:
HiSeq2500 PE250 mode + MiniION/PromethION (93Gb,~200X)+ Fa'afanua mata'utia (DLE-1+BspQ1)
Laulau 1 Fa'atusatusaga o Musa acuminata (DH-Pahang) fa'apotopotoga genome
Ata 2 Musa genomes fa'atusa fausaga fausaga
3rdPhaeodactylum tricornutum genome3
Igoa: Telomere-to-telomere genome assembly ofP
haeodactylum tricornutum
Doi:https://doi.org/10.1101/2021.05.04.442596
Taimi Fa'asalalau: Me 04, 2021
Inisetiute: Iunivesite i Sisifo, Kanata
Meafaitino
Phaeodactylum tricornutum(Aganuu Aoina o Algae ma Protozoa CCAP 1055/1)
Fa'asologa o fuafuaga ma fa'amaumauga:
1 Oxford Nanopore miniION sela sela + o le 2×75 fa'ai'uga fa'ai'uga ogatotonu o fafo NextSeq 550 tamoe
Ata 3 Fa'asologa o galuega mo le telomere-to-telomere genome assembly
4thTagata CHM13 genome4
Ulutala: Le fa'asologa atoa o se genome o le tagata
Doi:https://doi.org/10.1101/2021.05.26.445798
Taimi Fa'asalalau: Me 27, 2021
Inisetiute: National Institutes of Health (NIH), ISA
Mea: laina sela CHM13
Fa'asologa o fuafuaga ma fa'amaumauga:
30× PacBio circular consensus sequencing (HiFi) , 120× Oxford Nanopore ultra-long read sequencing , 100× Illumina PCR-Free sequencing (ILMN) , 70× Illumina / Arima Genomics Hi-C (Hi-C), BioNano maps optical, ma Strand-seq
Laulau 2 Fa'atusatusaga o GRCh38 ma T2T-CHM13 fa'apotopotoga genome tagata
Fa'asinomaga
1. Sergey Nurk et al.Le fa'asologa atoa o se genome tagata.bioRxiv 2021.05.26.445798;fai:https://doi.org/10.1101/2021.05.26.445798
2. Caroline Belser et al.Telomere-i-telomere gapless chromosomes o fa'i fa'aogaina le fa'asologa o le nanopore.bioRxiv 2021.04.16.440017;fai:https://doi.org/10.1101/2021.04.16.440017
3. Daniel J. Giguere et al.Telomere-i-telomere genome fa'apotopotoga o Phaeodactylum tricornutum.bioRxiv 2021.05.04.442596;fai:https://doi.org/10.1101/2021.05.04.442596
4.Jia-Ming Pese et al.Fonotaga ma Fa'amaoniaga o Genomes Fa'asinoga e lua Gap-free mo Xian/indica Rice Fa'aalia Malamalamaga i La'au Centromere Architecture.bioRxiv 2020.12.24.424073;fai:https://doi.org/10.1101/2020.12.24.424073
Taimi meli: Ian-06-2022