T2T GENOME ASSEMBLY, GAP dawb genome
1stOb hom Rice Genomes1
Lub npe: Kev sib dhos thiab kev lees paub ntawm Ob qhov sib txawv-dawb siv Genomes rau Xian / indica Rice Qhia Pom Kev Pom Zoo rau cog Centromere Architecture
Doi:https://doi.org/10.1101/2020.12.24.424073
Hnub Kawg Ua Ntawv Thov: Lub Ib Hlis 01, 2021.
Lub koom haum: Huazhong Agricultural University, Tuam Tshoj
Khoom siv
O. sativa xian/indicanplej ntau yam 'Zhenshan 97 (ZS97)' thiab 'Minghui 63 (MH63)
Sequencing tswv yim
NGS nyeem + HiFi nyeem + CLR nyeem + BioNano + Hi-C
Cov ntaub ntawv:
ZS97: 8.34 Gb (~23x) HiFi nyeem + 48.39 Gb (~131x) CLR nyeem + 25 Gb (~ 69x) NGS + 2 BioNano Irys hlwb
MH63: 37.88 Gb (~103x) HiFi nyeem + 48.97 Gb (~132x) CLR nyeem + 28 Gb (~76x) NGS + 2 BioNano Irys hlwb
Daim duab 1 Ob qhov sib txawv-dawb genomes ntawm nplej (MH63 thiab ZS97)
2ndTxiv tsawb Genome2
Title: Telomere-to-telomere gapless chromosomes ntawm txiv tsawb siv nanopore sequencing
Doi:https://doi.org/10.1101/2021.04.16.440017
Hnub Kawg Ua Ntawv Thov: Plaub Hlis 17, 2021.
Lub koom haum: Université Paris-Saclay, Fabkis
Khoom siv
Ob chav haploidMusa acuminataua sppmalaccensis(DH-Paj)
Sequencing lub tswv yim thiab cov ntaub ntawv:
HiSeq2500 PE250 hom + MinION / PromethION (93Gb, ~ 200X) + Optical map (DLE-1 + BspQ1)
Table 1 Kev sib piv ntawm Musa acuminata (DH-Pahang) genome assemblies
Daim duab 2 Musa genomes architecture sib piv
3rdPhaeodactylum tricornutum genome3
Title: Telomere-to-telomere genome assembly ofP
heodactylum tricornutum
Doi:https://doi.org/10.1101/2021.05.04.442596
Hnub Kawg Ua Ntawv Thov: May 04, 2021
Lub koom haum: Western University, Canada
Khoom siv
Phaeodactylum tricornutum(Culture Collection of Algae thiab Protozoa CCAP 1055/1)
Sequencing lub tswv yim thiab cov ntaub ntawv:
1 Oxford Nanopore minION ntws ntawm tes + 2 × 75 khub-kawg nruab nrab tso zis NextSeq 550 khiav
Daim duab 3 Workflow rau telomere-to-telomere genome los ua ke
4thHuman CHM13 genome4
Title: Kev ua tiav ntawm tib neeg genome
Doi:https://doi.org/10.1101/2021.05.26.445798
Hnub Kawg Ua Ntawv Thov: May 27, 2021
Lub koom haum: National Institutes of Health (NIH), USA
Khoom siv: cell kab CHM13
Sequencing lub tswv yim thiab cov ntaub ntawv:
30 × PacBio ncig kev pom zoo ua ntu zus (HiFi), 120 × Oxford Nanopore ultra-ntev nyeem sequencing, 100 × Illumina PCR-Free sequencing (ILMN), 70 × Illumina / Arima Genomics Hi-C (Hi-C), BioNano thiab Strand-seq
Table 2 Kev sib piv ntawm GRCh38 thiab T2T-CHM13 tib neeg genome sib dhos
Kev siv
1. Sergey Nurk et al.Ua tiav ib theem zuj zus ntawm tib neeg genome.bioRxiv 2021.05.26.445798;doi:https://doi.org/10.1101/2021.05.26.445798
2. Caroline Belser et al.Telomere-to-telomere gapless chromosomes ntawm txiv tsawb siv nanopore sequencing.bioRxiv 2021.04.16.440017;doi:https://doi.org/10.1101/2021.04.16.440017
3.Daniel J. Giguere et al.Telomere-to-telomere genome los ua ke ntawm Phaeodactylum tricornutum.bioRxiv 2021.05.04.442596;doi:https://doi.org/10.1101/2021.05.04.442596
4.Jia-Ming Song et al.Kev sib dhos thiab kev lees paub ntawm Ob qhov sib txawv-dawb Reference Genomes rau Xian / indica Rice Qhia Kev Pom Zoo rau Cog Centromere Architecture.bioRxiv 2020.12.24.424073;doi:https://doi.org/10.1101/2020.12.24.424073
Post lub sij hawm: Jan-06-2022