T2T INDIBANO YEGENOME, GAP FREE GENOME
1stIiGenome ezimbini zeRayisi1
Isihloko: INdibano kunye nokuQinisekiswa kweeGenomes ezimbini zeReferensi eziNgenaMpahla zeXian/indica Rice ityhila iiMfihlo kwiPlant Centromere Architecture
I-Doi:https://doi.org/10.1101/2020.12.24.424073
Ixesha elimiselweyo: NgoJanuwari 01, 2021.
Iziko: Huazhong Agricultural University, China
Izinto eziphathekayo
O. sativa xian/indicaiintlobo zerayisi 'Zhenshan 97 (ZS97)' kunye 'Minghui 63 (MH63)
Isicwangciso sokulandelelana
I-NGS iyafunda + i-HiFi ifundeka + i-CLR ifundeka + i-BioNano + i-Hi-C
Idatha:
I-ZS97: 8.34 Gb (~23x) i-HiFi ifunda + 48.39 Gb (~131x ) i-CLR ifundeka + 25 Gb (~69x) NGS + 2 iiseli ze-BioNano Irys
MH63: 37.88 Gb (~103x) i-HiFi ifundeka + 48.97 Gb (~132x) i-CLR ifundeka + 28 Gb(~76x) NGS + 2 iiseli zeBioNano Irys
Umzobo 1 Iigenomes ezimbini zerayisi (MH63 kunye neZS97)
2ndBanana Genome2
Isihloko: I-Telomere-to-telomere gapless chromosomes yebhanana isebenzisa ulandelelwano lwe-nanopore
I-Doi:https://doi.org/10.1101/2021.04.16.440017
Ixesha elibekiweyo: ngo-Epreli 17, 2021.
Iziko: IYunivesithi yaseParis-Saclay, eFransi
Izinto eziphathekayo
I-haploid ephindwe kabiniMusa acuminatasppisifo somhlaza(DH-Pahang)
Isicwangciso solandelelwano kunye nedatha:
Imowudi yeHiSeq2500 PE250 + MinION/ PromethION (93Gb, ~200X )+ Imephu yokubona (DLE-1+BspQ1)
Itheyibhile 1 Ukuthelekiswa kweendibano zegenome zikaMusa acuminata (DH-Pahang).
Umzobo 2 Musa genomes uthelekiso lwezakhiwo
3rdI-Phaeodactylum tricornutum genome3
Isihloko: I-Telomere-to-telomere genome assembly ofP
haeodactylum tricornutum
I-Doi:https://doi.org/10.1101/2021.05.04.442596
Ixesha elibekiweyo: ngoMeyi 04, 2021
Iziko: IYunivesithi yaseNtshona, eCanada
Izinto eziphathekayo
Phaeodactylum tricornutum(Ukuqokelelwa kweNkcubeko yeAlgae kunye neProtozoa CCAP 1055/1)
Isicwangciso solandelelwano kunye nedatha:
I-1 Oxford Nanopore mini flow flow cell + i-2 × 75 idityanisiwe-yesiphelo phakathi kwemveliso NextSeq 550 run
Umzobo 3 Ukuhamba komsebenzi kwindibano yegenome ye-telomere-to-telomere
4thI-CHM13 yegenome yomntu4
Isihloko: Ulandelelwano olupheleleyo lwegenome yomntu
I-Doi:https://doi.org/10.1101/2021.05.26.445798
Ixesha elibekiweyo: ngoMeyi 27, 2021
Iziko: Iziko leSizwe lezeMpilo (NIH), eU.SA
Izinto eziphathekayo: umgca weseli CHM13
Isicwangciso solandelelwano kunye nedatha:
30 × PacBio ulandelelwano lwesetyhula setyhula (HiFi) , 120 × Oxford Nanopore ultra-long read sequencing , 100 × Illumina PCR-Free sequencing (ILMN) , 70 × Illumina / Arima Genomics Hi-C (Hi-C), i-Bionos kunye neStrand-seq
Itheyibhile yesi-2 Uthelekiso lwe-GRCh38 kunye ne-T2T-CHM13 yeendibano zegenome yabantu
Isalathiso
1.USergey Nurk et al.Ulandelelwano olupheleleyo lwegenome yomntu.bioRxiv 2021.05.26.445798;doi:https://doi.org/10.1101/2021.05.26.445798
2.Caroline Belser et al.I-telomere-to-telomere gapless chromosomes yebhanana isebenzisa i-nanopore sequencing.bioRxiv 2021.04.16.440017;doi:https://doi.org/10.1101/2021.04.16.440017
3.Daniel J. Giguere et al.Indibano ye-Telomere-to-telomere genome ye-Phaeodactylum tricornutum.bioRxiv 2021.05.04.442596;doi:https://doi.org/10.1101/2021.05.04.442596
4.Jia-Ming Ingoma et al.INdibano kunye nokuQinisekiswa kweeGenomes ezimbini zeReferensi ye-Xian/indica iRice ityhila iiNgcaciso kwiPlant Centromere Architecture.bioRxiv 2020.12.24.424073;doi:https://doi.org/10.1101/2020.12.24.424073
Ixesha lokuposa: Jan-06-2022