Ubwihindurize
Inyungu za serivisi
Takagi n'abandi.,Ikinyamakuru cyibimera, 2013
ØKugereranya amoko atandukanye igihe n'umuvuduko ukurikije itandukaniro kurwego rwa nucleotide na aside amine
ØKugaragaza isano yizewe ya phylogeneque hagati yubwoko hamwe ningaruka ntoya yubwihindurize hamwe nubwihindurize
ØKubaka amahuza hagati yimiterere yimiterere na fenotipike kugirango ugaragaze ingirabuzimafatizo
ØKugereranya ubwoko butandukanye, bugaragaza ubushobozi bwihindagurika bwibinyabuzima
ØIgihe cyihuta
ØUbunararibonye bunini: BMK imaze imyaka isaga 12 ifite ubunararibonye mu baturage no mu mishinga ijyanye n’ubwihindurize, ikubiyemo amoko amagana, n'ibindi kandi itanga umusanzu mu mishinga isaga 80 yo mu rwego rwo hejuru yasohotse mu itumanaho ry’ibidukikije, Ibimera bya Molecular, Ikinyamakuru cy’ibinyabuzima cya Biotechnologiya, n'ibindi.
Ibisobanuro bya serivisi
Ibikoresho:
Mubisanzwe, byibuze bitatu-byabaturage (urugero: ubwoko buto cyangwa imirongo) birasabwa.Buri cyiciro cyabaturage kigomba kuba kitari munsi yabantu 10 (Ibimera ＞ 15, birashobora kugabanuka kubinyabuzima bidasanzwe).
Ingamba zikurikirana:
* WGS irashobora gukoreshwa kubinyabuzima bifite genome yo mu rwego rwo hejuru, mugihe SLAF-Seq ikoreshwa kubinyabuzima haba bifite cyangwa bidafite genome, cyangwa genome yerekana ubuziranenge.
| Bikoreshwa mubunini bwa genome | WGS | SLAF-Tags (×10,000) |
| ≤ 500 Mb | ≥ 5 × / umuntu ku giti cye | 10 |
| 500 Mb - 1.5 Gb | 20 - 30 | |
| 1.5 Gb - 3 Gb | 30 - 40 | |
| Genome nini cyangwa igoye | 40 cyangwa irenga |
Isesengura rya bioinformatics
üIsesengura ryubwihindurize
üGuhitamo guhitamo
üUrujya n'uruza
üAmateka ya demokarasi
üIgihe cyo gutandukana
Icyitegererezo gisabwa no gutanga
Icyitegererezo gisabwa:
| icyitegererezo cya gDNA | Icyitegererezo |
| Kwibanda: ≥30 ng / μl | Ibimera: 1-2 g |
| Umubare: ≥2 μg (Inkingi ≥15 μl) | Inyamaswa: 0.5-1 g |
| Isuku: OD260 / 280 = 1.6-2.5 | Amaraso yose: 1.5 ml |
Akazi ka serivisi
Igishushanyo mbonera
Icyitegererezo
Kubaka isomero
Urukurikirane
Isesengura ryamakuru
Serivisi nyuma yo kugurisha
*Ibisubizo byerekanwa hano byose biva muri genomes yatangajwe na Biomarker Technologies
1.Isesengura ryubwihindurize ririmo kubaka igiti cya phylogeneque, imiterere yabaturage na PCA hashingiwe ku moko atandukanye.
Igiti cya phylogeneque kigereranya isano ya tagisi nubwihindurize hagati yubwoko hamwe nabakurambere.
PCA igamije kwiyumvisha hafi hagati yabaturage.
Imiterere yabaturage yerekana ko hariho ubwoko butandukanye bwubwoko butandukanye bwa allele.
Chen, n'abandi.al.,PNAS, 2020
2.Guhitamo neza
Guhitamo gutoranya bivuga inzira yatoranijwe kurubuga rwiza kandi inshuro zimbuga zidafite aho zibogamiye zikiyongera kandi nimbuga zidafunze ziragabanuka, bigatuma kugabanuka kwakarere.
Kugaragaza genome-mugace utoranyirijwe hamwe bitunganyirizwa mukubara urutonde rwabaturage （π ， Fst, Tajima's D) ya SNPs zose mumadirishya anyerera (100 Kb) kumurongo runaka (10 Kb).
Nucleotide itandukanye (π）
Tajima's D.
Icyerekezo cyo gukosora (Fst)
Wu, n'abandi.al.,Igihingwa cya molekile, 2018
3.Gen Flow
Wu, n'abandi.al.,Igihingwa cya molekile, 2018
4.Amateka ya demokarasi
Zhang, n'abandi.al.,Ibidukikije bya Kamere & Ubwihindurize, 2021
5.Igihe cyo gutandukana
Zhang, n'abandi.al.,Ibidukikije bya Kamere & Ubwihindurize, 2021
Urubanza rwa BMK
Ikarita ihindagurika ya genomic itanga ubushishozi bushingiye kumiterere yimiterere yubushinwa (Brassica rapa ssp. Pekinensis)
Byatangajwe: Igihingwa cya molekile, 2018
Ingamba zikurikirana:
Igisubizo: ubujyakuzimu bukurikirana: 10 ×
Ibisubizo by'ingenzi
Muri ubu bushakashatsi, amashu 194 yo mu Bushinwa yatunganijwe kugirango yongere akurikirane hamwe n’ubujyakuzimu bwa 10 ×, byatanze SNPs 1,208.499 na InDels 416.070.Isesengura rya phylogeneque kuriyi mirongo 194 ryerekanye ko iyi mirongo ishobora kugabanywamo ecotypes eshatu, impeshyi, icyi nimpeshyi.Byongeye kandi, imiterere yabaturage hamwe nisesengura rya PCA byerekanaga ko imyumbati yabashinwa yakomotse kuri cabage yumuhindo i Shandong, mubushinwa.Ibyo byaje kumenyekana muri Koreya no mu Buyapani, byambukiranya imirongo yaho kandi ubwoko bumwebumwe bwatinze gutondekwa mubushinwa hanyuma amaherezo biba imyumbati yubushinwa.
Gusikana kuri genome-yose ku mbuto zo mu Bushinwa hamwe na keleti zo mu gihe cyizuba byatoranijwe byerekanaga genomique 23 yagiye mu guhitamo gukomeye, bibiri muri byo bikaba byari byuzuyemo akarere kayobora igihe gishingiye kuri QTL-mapping.Utu turere twombi wasangaga turimo genes zingenzi zigenga indabyo, BrVIN3.1 na BrFLC1.Izi genes zombi zongeye kwemezwa ko zigira uruhare muguhindura igihe hakoreshejwe ubushakashatsi bwakozwe na transgenji.
 Isesengura ryimiterere yabaturage kuri cabage |  Ibisobanuro bya genetike kubijyanye no gutoranya imyumbati |
Tongbing, n'abandi.“Ikarita ya Genomic Variation itanga ubushishozi ku miterere ya genetike y’imyumbati yo mu Bushinwa (Brassica rapa ssp.pekinensis).”Ibimera bya molekile,11 (2018): 1360-1376.
